ErbB2/Her2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-29624

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to C-terminal portion of human HER2 protein (between residues 1100-1200) [UniProt P04626]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ErbB2/Her2 Antibody - BSA Free

Western Blot: ErbB2/Her2 AntibodyBSA Free [NBP2-29624]

Western Blot: ErbB2/Her2 AntibodyBSA Free [NBP2-29624]

Western Blot: ErbB2/HER2 Antibody [NBP2-29624] - Western blot analysis of ErbB2/HER2 in SKOV3 cell lysate.
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody - BSA Free [NBP2-29624]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody - BSA Free [NBP2-29624]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP2-29624] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:50 dilution of ErbB2/HER2 antibody (NBP2-29624) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This ErbB3 antibody generated a diffused cytoplasmic with some membrane and nuclear staining in the cancer cells, endothelial cells as well as in the tumor stromal cells. The cells in the cores of some tumor areas were negative for ErbB2.
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody - BSA Free [NBP2-29624]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody - BSA Free [NBP2-29624]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP2-29624] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:50 dilution of ErbB2/HER2 antibody (NBP2-29624) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This ErbB2 antibody generated a diffused cytoplasmic with some membrane and nuclear staining in the cancer cells, endothelial cells as well as in the tumor stromal cells. Staining was performed by Histowiz.

Applications for ErbB2/Her2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ErbB2/Her2

Her-2 (also known as c-erbB-2 or p185 Her-2/neu) is a 185 kDa type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. Overexpression of Her-2 is found in human breast cancer and has also prognostic significance in patients with ovarian, gastric and salivary gland cancers. Recombinant human Her-2 is a 84-90 kDa protein containing 630 amino acid residues. Recombinant human Her-2 encodes amino acid 23 - 652 of the full length protein.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Entrez Gene IDs

2064 (Human); 2064 (Human)

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ErbB2/Her2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ErbB2/Her2 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ErbB2/Her2 Antibody - BSA Free

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  • Q: I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?

    A: A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

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