ErbB3/Her3 Antibody (6F6) - BSA Free

Novus Biologicals | Catalog # NBP3-26599

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6F6 expressed in HEK293

Format

BSA Free
View all ErbB3/Her3 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human ErbB3/Her3 [UniProt P21860]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit ErbB3/Her3 Antibody (6F6) - BSA Free (NBP3-26599) is a recombinant monoclonal antibody validated for use in ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ErbB3/Her3 Antibody (6F6) - BSA Free

ErbB3/Her3 Antibody (6F6)

Immunocytochemistry/Immunofluorescence: ErbB3/Her3 Antibody (6F6) [NBP3-26599] -

Immunocytochemistry/Immunofluorescence: ErbB3/Her3 Antibody (6F6) [NBP3-26599] - Staining of MCF7 Cells with ErbB3/Her3 Antibody (6F6) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for ErbB3/Her3 Antibody (6F6) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: ErbB3/Her3

The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized.

Long Name

Receptor Tyrosine Protein Kinase ErbB3

Alternate Names

HER3

Gene Symbol

ERBB3

Additional ErbB3/Her3 Products

Product Documents for ErbB3/Her3 Antibody (6F6) - BSA Free

Certificate of Analysis

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Product Specific Notices for ErbB3/Her3 Antibody (6F6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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