ErbB4/Her4 Antibody (H4.77.16 (Ab77))

Novus Biologicals | Catalog # NB120-3104

Novus Biologicals
Discontinued Product
NB120-3104 has been discontinued. View all ErbB4/Her4 products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # H4.77.16 (Ab77)
View all ErbB4/Her4 Primary Antibodies »

Product Specifications

Immunogen

Extracellular fragment of recombinant human c-erbB-4/HER-4 oncoprotein

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Type I membrane protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ErbB4/Her4 Antibody (H4.77.16 (Ab77))

Western Blot: ErbB4/Her4 Antibody (H4.77.16 (Ab77)) [NB120-3104]

Western Blot: ErbB4/Her4 Antibody (H4.77.16 (Ab77)) [NB120-3104]

Western Blot: ErbB4/Her4 Antibody (H4.77.16) [NB120-3104] - Analysis of 25 ug of mouse brain cell lysates.
Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16 (Ab77)) [NB120-3104]

Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16 (Ab77)) [NB120-3104]

Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16) [NB120-3104] - Analysis of HER-4 in T47D cells (green) compared to an isotype control (blue).
Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16 (Ab77)) [NB120-3104]

Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16 (Ab77)) [NB120-3104]

Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16) [NB120-3104] - Analysis of HER-4 in MCF-7 cells (green) compared to an isotype control (blue).
Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16 (Ab77)) [NB120-3104]

Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16 (Ab77)) [NB120-3104]

Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16) [NB120-3104] - Analysis of HER-4 in Neuro-2a cells (green) compared to an isotype control (blue).

Applications for ErbB4/Her4 Antibody (H4.77.16 (Ab77))

Application
Recommended Usage

Flow Cytometry

0.5 ug

Immunocytochemistry/ Immunofluorescence

2 ug/ml

Immunoprecipitation

2 ug/mg

Western Blot

1:50 - 1:500
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID 28267150).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4) and 0.2% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ErbB4/Her4

C-erbB-4 is the fourth member of class I receptor kinase family (ErbB). Down regulation of ErbB-4 in estrogen receptor-positive cancer cells inhibits proliferation.

Long Name

Receptor Tyrosine Protein Kinase ErbB4

Alternate Names

HER4

Entrez Gene IDs

2066 (Human); 13867 (Mouse); 29496 (Rat)

Gene Symbol

ERBB4

UniProt

Q15303

Additional ErbB4/Her4 Products

Product Documents for ErbB4/Her4 Antibody (H4.77.16 (Ab77))

Certificate of Analysis

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Product Specific Notices for ErbB4/Her4 Antibody (H4.77.16 (Ab77))

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ErbB4/Her4 Antibody (H4.77.16 (Ab77))

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Protocols

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