ERCC1 Antibody (S08-7E3)

Name: ERCC1 Antibody (S08-7E3)
Brand: Novus Biologicals
SKU: NBP3-14929
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-14929

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-7E3 expressed in HEK293

View all ERCC1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human ERCC1 (Uniprot#: P07992)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERCC1 Antibody (S08-7E3)

Western Blot: ERCC1 Antibody (S08-7E3) [NBP3-14929]

Western Blot: ERCC1 Antibody (S08-7E3) [NBP3-14929] - Western blot detection of ERCC1 in K562, 3T3, Hela cell lysates using ERCC1 Rabbit mAb (1:1000 diluted). Predicted band size: 33kDa. Observed band size: 39kDa.

Immunocytochemistry/ Immunofluorescence: ERCC1 Antibody (S08-7E3) [NBP3-14929]

Immunocytochemistry/Immunofluorescence: ERCC1 Antibody (S08-7E3) [NBP3-14929] - Immunofluorescence of ERCC1 (green) in Hela cells using ERCC1 Rabbit mAb at dilution 1/200, and DAPI (blue)

Applications for ERCC1 Antibody (S08-7E3)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20-1/100

Western Blot

1/1000-1/5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERCC1

The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1 XPF complex makes the 5'incision. Elevated levels of ERCC1 have also been reported in Cisplatin resistant cells.

Long Name

Excision Repair Cross-complementing 1

Alternate Names

COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20

Gene Symbol

ERCC1

Additional ERCC1 Products

Product Documents for ERCC1 Antibody (S08-7E3)

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Certificate of Analysis

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Product Specific Notices for ERCC1 Antibody (S08-7E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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