Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4H7
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Product Specifications
Immunogen
Full length human recombinant protein of human ERG(NP_891548) produced in HEk293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
53.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse ERG Antibody (OTI4H7) (NBP2-45520) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ERG Antibody (OTI4H7)
![Western Blot: ERG Antibody (OTI4H7) [NBP2-45520]](https://resources.rndsystems.com/images/products/ERG-Antibody-OTI4H7-Western-Blot-NBP2-45520-img0005.jpg "Western Blot: ERG Antibody (OTI4H7) [NBP2-45520]")
Western Blot: ERG Antibody (OTI4H7) [NBP2-45520]
Western Blot: ERG Antibody (OTI4H7) [NBP2-45520] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERG.![Immunohistochemistry: ERG Antibody (OTI4H7) [NBP2-45520]](https://resources.rndsystems.com/images/products/ERG-Antibody-OTI4H7-Immunohistochemistry-NBP2-45520-img0004.jpg "Immunohistochemistry: ERG Antibody (OTI4H7) [NBP2-45520]")
Immunohistochemistry: ERG Antibody (OTI4H7) [NBP2-45520]
Immunohistochemistry: ERG Antibody (OTI4H7) [NBP2-45520] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Western Blot: ERG Antibody (OTI4H7) [NBP2-45520]](https://resources.rndsystems.com/images/products/ERG-Antibody-OTI4H7-Western-Blot-NBP2-45520-img0006.jpg "Western Blot: ERG Antibody (OTI4H7) [NBP2-45520]")
Western Blot: ERG Antibody (OTI4H7) [NBP2-45520]
Western Blot: ERG Antibody (OTI4H7) [NBP2-45520] - Analysis of extracts (10ug) from 8 Human tissue (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).![Immunohistochemistry: ERG Antibody (OTI4H7) [NBP2-45520]](https://resources.rndsystems.com/images/products/ERG-Antibody-OTI4H7-Immunohistochemistry-NBP2-45520-img0002.jpg "Immunohistochemistry: ERG Antibody (OTI4H7) [NBP2-45520]")
Immunohistochemistry: ERG Antibody (OTI4H7) [NBP2-45520]
Immunohistochemistry: ERG Antibody (OTI4H7) [NBP2-45520] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: ERG Antibody (OTI4H7) [NBP2-45520]](https://resources.rndsystems.com/images/products/ERG-Antibody-OTI4H7-Immunohistochemistry-NBP2-45520-img0003.jpg "Immunohistochemistry: ERG Antibody (OTI4H7) [NBP2-45520]")
Immunohistochemistry: ERG Antibody (OTI4H7) [NBP2-45520]
Immunohistochemistry: ERG Antibody (OTI4H7) [NBP2-45520] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunofluorescence: ERG Antibody (OTI4H7) [NBP2-45520]](https://resources.rndsystems.com/images/products/ERG-Antibody-OTI4H7-Immunofluorescence-NBP2-45520-img0001.jpg "Immunofluorescence: ERG Antibody (OTI4H7) [NBP2-45520]")
Immunofluorescence: ERG Antibody (OTI4H7) [NBP2-45520]
Immunofluorescence: ERG Antibody (OTI4H7) [NBP2-45520] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ERG.Applications for ERG Antibody (OTI4H7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Erg
Long Name
v-Ets Erythroblastosis Virus E26 Oncogene Homolog
Alternate Names
Erg-3, p55
Gene Symbol
ERG
Additional Erg Products
Product Documents for ERG Antibody (OTI4H7)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ERG Antibody (OTI4H7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for ERG Antibody (OTI4H7)
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Q: I am looking for an antibody that can IP Erg protein and doesn't cross react with Fli protein.
A: I have spoken to the lab and they have informed me that actually that the antibody (catalog #NBP1-95857) is confirmed not to cross react with Fli1 protein so it should be a viable choice for you in your experiments on Erg.
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