ERG Antibody

Novus Biologicals | Catalog # NB100-2472

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Erg Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PNTRLPTSHMPSH corresponding to C-Terminus according to NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1|.

Epitope

C-PNTRLPTSHMPSH

Specificity

This antibody is expected to recognise all reported isoforms (NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat ERG Antibody (NB100-2472) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-ERG Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ERG Antibody

Western Blot: ERG Antibody [NB100-2472]

Western Blot: ERG Antibody [NB100-2472]

Western Blot: ERG Antibody [NB100-2472] - Staining of MOLT4 lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: ERG Antibody [NB100-2472]

Immunocytochemistry/ Immunofluorescence: ERG Antibody [NB100-2472]

Immunocytochemistry/Immunofluorescence: ERG Antibody [NB100-2472] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: ERG Antibody [NB100-2472]

Flow Cytometry: ERG Antibody [NB100-2472]

Flow Cytometry: ERG Antibody [NB100-2472] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for ERG Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:6000

Western Blot

0.1 - 0.3 ug/mL

Reviewed Applications

Read 1 review rated 1 using NB100-2472 in the following applications:

Flow Cytometry Panel Builder

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Erg

Gene ID(s): 2078 (human)

Long Name

v-Ets Erythroblastosis Virus E26 Oncogene Homolog

Alternate Names

Erg-3, p55

Entrez Gene IDs

2078 (Human); 13876 (Mouse); 170909 (Rat)

Gene Symbol

ERG

UniProt

NP_891548.1 (Human)

Additional Erg Products

Product Documents for ERG Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ERG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ERG Antibody (1)

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Customer Images

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  • ERG Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Rat Retina and mouse retina
    Species: Mouse and Rat
    Verified Customer | Posted 03/13/2023
    Picture Summary, Whole mounts rat and mouse retina, gt anti ERG, 20x
    ERG Antibody NB100-2472
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ERG Antibody

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  • Q: I am looking for an antibody that can IP Erg protein and doesn't cross react with Fli protein.

    A: I have spoken to the lab and they have informed me that actually that the antibody (catalog #NBP1-95857) is confirmed not to cross react with Fli1 protein so it should be a viable choice for you in your experiments on Erg.

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