ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-23530
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to residues around Thr202, Tyr204 of Human phospho-ERK1/ERK2
Modification
p Thr202, p Tyr204
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.
Scientific Data Images for ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free
Immunohistochemistry-Paraffin: ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free [NBP3-23530] -
Immunohistochemistry-Paraffin: ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free [NBP3-23530] - Immunochemical staining of human ERK1/ERK21 in human breast carcinoma with NBP3-23530 at 1:100 dilution, formalin-fixed paraffin embedded sections.Immunohistochemistry-Paraffin: ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free [NBP3-23530] -
Immunohistochemistry-Paraffin: ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free [NBP3-23530] - Immunochemical staining of human ERK1/ERK2 in human lung cancer with NBP3-23530 at 1:100 dilution, formalin-fixed paraffin embedded sections.Western Blot: ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free [NBP3-23530] -
Western Blot: ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free [NBP3-23530] - Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with Calyculin A (100nM, 30 min; +), using NBP3-23530 at 1:1000 dilution.Applications for ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ERK1/ERK2
Long Name
Extracellular Signal-regulated Kinase 1/2
Alternate Names
ERK1, ERK-1, ERT2, Extracellular signal-regulated kinase 1, extracellular signal-related kinase 1, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 kinase, MAPK 1, Microtubule-associated protein 2 kinase, mitogen-activated protein kinase 3, p44erk1, p44-ERK1, p44mapk, p44-MAPK, PRKM3
Gene Symbol
MAPK3
Additional ERK1/ERK2 Products
Product Documents for ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free
Product Specific Notices for ERK1/ERK2 [p Thr202, p Tyr204] Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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