ERK3/MAPK6 Antibody (1G6) - Azide and BSA Free

Novus Biologicals | Catalog # H00005597-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G6

Format

Azide and BSA Free
View all ERK3 Primary Antibodies »

Product Specifications

Immunogen

MAPK6 (AAH35492, 612 a.a. ~ 721 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EVRKDEQVEKENTYTSYLDKFFSRKEDTEMLETEPVEDGKLGERGHEEGFLNNSGEFLFNKQLESIGIPQFHSPVGSPLKSIQATLTPSAMKSSPQIPHQTYSSILKHLN

Specificity

MAPK6 - mitogen-activated protein kinase 6

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ERK3/MAPK6 Antibody (1G6) - Azide and BSA Free

Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01]

Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01]

Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of MAPK6 expression in transfected 293T cell line by MAPK6 monoclonal antibody (M01), clone 1G6.Lane 1: MAPK6 transfected lysate(82.7 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (1G6) [H00005597-M01]

Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (1G6) [H00005597-M01]

Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of monoclonal antibody to MAPK6 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01]

Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01]

Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of MAPK6 over-expressed 293 cell line, cotransfected with MAPK6 Validated Chimera RNAi ( Cat # H00005597-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK6 monoclonal antibody (M01) clone 1G6 (Cat # H00005597-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Detection limit for recombinant GST tagged MAPK6 is approximately 0.03ng/ml as a capture antibody.

Applications for ERK3/MAPK6 Antibody (1G6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ERK3

The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Long Name

Extracellular Signal-regulated Kinase 3

Alternate Names

MAPK6, p97MAPK

Entrez Gene IDs

5597 (Human)

Gene Symbol

MAPK6

OMIM

602904 (Human)

UniProt

AAH35492 (Human)

Additional ERK3 Products

Product Documents for ERK3/MAPK6 Antibody (1G6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ERK3/MAPK6 Antibody (1G6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

GDNF Family
Intracellular Kinases

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Protocols

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FAQs

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