Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2D7

Format

Azide and BSA Free
View all ERK4 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MAPK4(NP_002738) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

65.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free

Western Blot: ERK4/MAPK4 Antibody (OTI2D7)Azide and BSA Free [NBP2-71240]

Western Blot: ERK4/MAPK4 Antibody (OTI2D7)Azide and BSA Free [NBP2-71240]

Western Blot: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK4.
Immunocytochemistry/ Immunofluorescence: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240]

Immunocytochemistry/ Immunofluorescence: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240]

Immunocytochemistry/Immunofluorescence: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ERK4/MAPK4.
Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240]

Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240]

Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: ERK4/MAPK4 Antibody (OTI2D7)Azide and BSA Free [NBP2-71240]

Western Blot: ERK4/MAPK4 Antibody (OTI2D7)Azide and BSA Free [NBP2-71240]

Western Blot: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240] - Analysis of extracts (10ug) from 8 Human tissue (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240]

Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240]

Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240]

Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240]

Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240]

Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240]

Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240]

Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240]

Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240]

Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240]

Immunohistochemistry: ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free [NBP2-71240] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ERK4

MAPK4 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Long Name

Extracellular Signal-regulated Kinase A

Alternate Names

MAPK4, p63Mapk, PRKM4

Gene Symbol

MAPK4

Additional ERK4 Products

Product Documents for ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ERK4/MAPK4 Antibody (OTI2D7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

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Protocols

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FAQs

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