ERO1LB Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83961

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (92%), Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ERO1LB Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NLKRPCPFWAEDGHCSIKDCHVEPCPESKIPVGIKAGHSNKYLKMANNTKELEDCEQANKLGAINSTLSNQSKEAFIDWARYDDSRDHFCELDDE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERO1LB Antibody - BSA Free

Western Blot: ERO1LB Antibody [NBP1-83961]

Western Blot: ERO1LB Antibody [NBP1-83961]

Western Blot: ERO1LB Antibody [NBP1-83961] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunohistochemistry-Paraffin: ERO1LB Antibody [NBP1-83961]

Immunohistochemistry-Paraffin: ERO1LB Antibody [NBP1-83961]

Immunohistochemistry-Paraffin: ERO1LB Antibody [NBP1-83961] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.

Applications for ERO1LB Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERO1LB

Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. Required for the folding of cell, thereby being a source of oxidative stress

Alternate Names

DKFZp779C1042, DKFZp779I0141, EC 1.8.4, EC 1.8.4.-, Endoplasmic oxidoreductin-1-like protein B, ERO1-L(beta), ERO1-L-beta, ERO1-like beta (S. cerevisiae), ERO1-like protein beta, FLJ11003, oxidoreductin-1-L-beta

Gene Symbol

ERO1B

Additional ERO1LB Products

Product Documents for ERO1LB Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ERO1LB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ERO1LB Antibody - BSA Free

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Protocols

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