Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 9E9

Format

BSA Free
View all ERp57/PDIA3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human ERp57/PDIA3 (25-505aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Novus Biologicals Mouse ERp57/PDIA3 Antibody (9E9) - BSA Free (NBP2-59431) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ERp57/PDIA3 Antibody (9E9) - BSA Free

Western Blot: ERp57/PDIA3 Antibody (9E9)BSA Free [NBP2-59431]

Western Blot: ERp57/PDIA3 Antibody (9E9)BSA Free [NBP2-59431]

Western Blot: ERp57/PDIA3 Antibody (9E9) [NBP2-59431] - Lane 1: HeLa cell lysates, Lane 2: HepG2 cell lysates, Lane 3: NIH3T3 cell lysates, Lane 4: Raw 264.7 cell lysates, Lane 5: LNCaP cell lysates
Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (9E9) - BSA Free [NBP2-59431]

Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (9E9) - BSA Free [NBP2-59431]

Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (9E9) [NBP2-59431] - Analysis of PDIA3 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDIA3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for ERp57/PDIA3 Antibody (9E9) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERp57/PDIA3

ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glucosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).

Long Name

Endoplasmic Reticulum Resident Protein 57

Alternate Names

ERp60, GRP58, PDIA3

Gene Symbol

PDIA3

Additional ERp57/PDIA3 Products

Product Documents for ERp57/PDIA3 Antibody (9E9) - BSA Free

Certificate of Analysis

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Product Specific Notices for ERp57/PDIA3 Antibody (9E9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

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Protocols

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