Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all ETHE1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human ETHE1 (Accession#: O95571; Leu13-Ala254)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ETHE1 Antibody - Azide and BSA Free

ETHE1 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: ETHE1 Antibody [NBP3-00355] -

Immunohistochemistry-Paraffin: ETHE1 Antibody [NBP3-00355] - Immunochemical staining of human ETHE1 in human colon carcinoma with NBP3-00355 (1:10000, formalin-fixed paraffin embedded sections).
ETHE1 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: ETHE1 Antibody [NBP3-00355] -

Immunohistochemistry-Paraffin: ETHE1 Antibody [NBP3-00355] - Immunochemical staining of human ETHE1 in human pancreas with NBP3-00355 (1:10000, formalin-fixed paraffin embedded sections).
ETHE1 Antibody - Azide and BSA Free

Western Blot: ETHE1 Antibody [NBP3-00355] -

Western Blot: ETHE1 Antibody [NBP3-00355] - NBP3-00355 at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HCT116 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:25 kDa (We are unsure as to the identity of these extra bands.)
ETHE1 Antibody - Azide and BSA Free

Immunoprecipitation: ETHE1 Antibody [NBP3-00355] -

Immunoprecipitation: ETHE1 Antibody [NBP3-00355] - ETHE1 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg HepG2 Whole Cell Lysate Lane C:0.5 mg Jurkat Whole Cell Lysate 2 uL NBP3-00355 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00355, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa
ETHE1 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: ETHE1 Antibody [NBP3-00355] -

Immunohistochemistry-Paraffin: ETHE1 Antibody [NBP3-00355] - Immunochemical staining of human ETHE1 in human appendix with NBP3-00355 (1:10000, formalin-fixed paraffin embedded sections).

Applications for ETHE1 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:2500-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ETHE1

ETHE1 encodes a protein that is expressed in the thyroid. [provided by RefSeq]

Alternate Names

D83198, EC 3.1.2.6, ethylmalonic encephalopathy 1, Ethylmalonic encephalopathy protein 1, Hepatoma subtracted clone one protein, HSCOEC 3.-, protein ETHE1, mitochondrial, YF13H12

Gene Symbol

ETHE1

Additional ETHE1 Products

Product Documents for ETHE1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ETHE1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ETHE1 Antibody - Azide and BSA Free

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Protocols

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FAQs

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