ETS1 associated protein II Antibody

Name: ETS1 associated protein II Antibody
Brand: Novus Biologicals
SKU: NBP1-46178
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-46178

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 50 and 100 of human Tyrosyl DNA Phosphodiesterase-2 using the numbering given in entry NP_057698.2 (GeneID 51567).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ETS1 associated protein II Antibody

Western Blot: ETS1 associated protein II Antibody [NBP1-46178]

Western Blot: ETS1 associated protein II Antibody [NBP1-46178] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-TDP2 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. TDP2 was also immunoprecipitated by another rabbit anti-TDP2 antibody, which recognizes a downstream epitope.

Immunohistochemistry: ETS1 associated protein II Antibody [NBP1-46178]

Immunohistochemistry: ETS1 associated protein II Antibody [NBP1-46178] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-TDP2 used at a dilution of 1:200 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit.

Applications for ETS1 associated protein II Antibody

Application

Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Application Notes

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ETS1 associated protein II

Ets proteins constitute a family of conserved sequence-specific DNA-binding proteins and function as transcription factors with roles in differentiation, lymphoid cell development, invasiveness and angiogenesis. A novel ETS1 interacting protein designated as ETS1-associated protein II (EAPII) interacts with ETS1 and other Ets proteins (ETS2 and FLI1) and is a novel ETS1 modulator.

Alternate Names

AD022, dJ30M3.3, EAP2, EAPII, EC 3.1.4.-, ETS1-associated protein 2, ETS1-associated protein II, MGC111021,5'-Tyr-DNA phosphodiesterase, MGC9099,5'-tyrosyl-DNA phosphodiesterase, TRAF and TNF receptor associated protein, TTRAPTRAF and TNF receptor-associated protein, Tyr-DNA phosphodiesterase 2, tyrosyl-DNA phosphodiesterase 2

Entrez Gene IDs

51567 (Human)

Gene Symbol

TDP2

UniProt

O95551 (Human)

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Product Specific Notices for ETS1 associated protein II Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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