ETS1 associated protein II Antibody [FITC]

Novus Biologicals | Catalog # NBP2-11492

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

The synthetic peptide taken from human Tyrosyl-DNAphosphodiesterase (TDP2) protein corresponding to amino acids spanning 335-356. The peptide was conserved in several other species. The selected peptide was post-synthetically modified to achieve highest antigenicity before coupling to carrier protein using hetero bifunctional cross linker for immunogen preparation

Reactivity Notes

Rat, Mouse and Human

Specificity

The antibody labels a strong band of TDP2 of 45kDa in TDP12 samples and in several cell lines. The antibody also labels a diffuse band of around 95 and 115kDa, the identity of these bands is not known but may represent TDP2 splice variants.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for ETS1 associated protein II Antibody [FITC]

Application
Recommended Usage

ELISA

1:500

Western Blot

1:500
Application Notes
TDP2 antibodies were tested in ELISA and western blotting applications

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ETS1 associated protein II

Tyrosyl DNA phosphodiesterase-2 (TDP2) activity involves TRAF and TNF receptor-associated protein (TTRAP), which are 5'-tyrosyl DNA phosphodiesterase (5'-TDP) that can cleave 5'-phosphotyrosyl bonds, which can be denoted as previously mentioned TDP2.(1) Topoisomerase II (Top2) activity involves these intermediaries which the topoisomerase is covalently bound to a DNA double-strand via 5'posphotyrsyl bond. These intermediates are normally transient; they can be stabilized by anti-tumor agents that act as Top2 poisons resulting in the induction of cytotoxic double-strand breaks. And they are implicated in the formation of site-specific translocations that are commonly associated with cancer patterns. Topoisomerases regulate DNA topology and play a key role to many aspects of chromosome metabolism. Their actions include transient cleavage of DNA, which if it occurs around sites of endogenous DNA damage or in the presence of topoisermase poisons, can resulting in abortive topoisomerase-induced DNA strand breaks (2). These breaks possess covalent linkage of the enzyme to the DNA termini by a 3' or 5'- phosphotyrosyl bond and are implicated in hereditary human disease, chromosomal instability and cancer. (2). It has been shown that TDP2-delete DT40 cells are highly sensitive to anticancer Top2 poison, etoposide, but are not hypersensitive to the Top 1 poison camptothecin, or the DNA-alkyating agent, methyl methanesulfonate. These results support an important mechanism for resistance to Top2-induced chromosome breakage and raise the likelihood that TDP2 is a significant factor in cancer development and treatment (1). The significance of liberating DNA termini from trapped topisomerase is shown by the progressive neurodegenerative disease observed in individuals possessing the mutation in tyrosyl-DNA phosodiesterase 1 (TDP1). A complementary human enzyme that cleaves 5'-phosphotyrosyl bonds has not been reported. An enzyme TTRAP a member of the Mg(2+)/Mn(2+)dependent family of phospdiesterases, whose cellular results in increased sensitivity toward topoisomerase-ii-induced DNA double strand breaks. TTRAP therefore is the first human 5'-tyrosyl DNA phophodiesterase to be identified, and it is suggested that this enzyme is denoted tyrosyl DNA phosphopdiesterease-2 (2).

Alternate Names

AD022, dJ30M3.3, EAP2, EAPII, EC 3.1.4.-, ETS1-associated protein 2, ETS1-associated protein II, MGC111021,5'-Tyr-DNA phosphodiesterase, MGC9099,5'-tyrosyl-DNA phosphodiesterase, TRAF and TNF receptor associated protein, TTRAPTRAF and TNF receptor-associated protein, Tyr-DNA phosphodiesterase 2, tyrosyl-DNA phosphodiesterase 2

Gene Symbol

TDP2

Additional ETS1 associated protein II Products

Product Documents for ETS1 associated protein II Antibody [FITC]

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Product Specific Notices for ETS1 associated protein II Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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