Exosome component 5 Antibody (V80P1C10*C) - BSA Free
Novus Biologicals | Catalog # NBP2-50173
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Clone # V80P1C10*C
Format
BSA Free
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Product Specifications
Immunogen
Peptide Sequence - KLLMSSTKGLY
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Exosome component 5 Antibody (V80P1C10*C) - BSA Free
Immunohistochemistry: Exosome component 5 Antibody (V80P1C10*C) - BSA Free [NBP2-50173]
Immunohistochemistry: Exosome component 5 Antibody (V80P1C10*C) [NBP2-50173] - Staining of FFPE hepatocellular carcinoma tissue sections using anti-CML28 [V80P1C10 C]. Cytoplasmic staining was observed.Flow Cytometry: Exosome component 5 Antibody (V80P1C10*C) - BSA Free [NBP2-50173]
Flow Cytometry: Exosome component 5 Antibody (V80P1C10*C) [NBP2-50173] - FACS staining with EXOSC5-Alexa Fluor 488 conjugated antibody (NBP2-50173AF488) in testicular germ cell tumor cell lines TCam-2, 2102EP, NCCIT, NT2/D1, JAR, JEG-3 and BeWo as well as control cell lines FS1 (Sertoli cells) and human fibroblasts. Image from verified customer review.Applications for Exosome component 5 Antibody (V80P1C10*C) - BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunohistochemistry
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
Positive control(s): Jurkat cell lysate.
Reviewed Applications
Read 1 review rated 4 using NBP2-50173 in the following applications:
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Exosome component 5
Alternate Names
CML28, exosome complex exonuclease RRP46, exosome component 5Chronic myelogenous leukemia tumor antigen 28, exosome component Rrp46, hRrp46p, MGC12901, p12BMGC111224, RRP41B, Rrp46p, RRP46Ribosomal RNA-processing protein 46
Gene Symbol
EXOSC5
Additional Exosome component 5 Products
Product Documents for Exosome component 5 Antibody (V80P1C10*C) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Exosome component 5 Antibody (V80P1C10*C) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for Exosome component 5 Antibody (V80P1C10*C) - BSA Free (1)
4 out of 5
1 Customer Rating
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Application: Flow CytometrySample Tested: 3 human cancer cell lines, fibroblasts, JAR human choriocarcinoma cell line, JEG-3 human epithelial choriocarcinoma cell line, BeWo human choriocarcinoma cell line, Germ cell cancer cell lines, Testicular germ cell cancer cell lines, Human testis and Testicular germ cell tumour cell linesSpecies: HumanVerified Customer | Posted 11/06/2018FACS staining with EXOSC5-A488 conjugated antibody (NBP2-50173-AF488) in testicular germ cell tumour cell lines TCam-2, 2102EP, NCCIT, NT2/D1, JAR, JEG-3 and BeWo as well as control cell lines FS1 (Sertoli cells) and human fibroblasts.Cells were fixated in 4% formaldehyde and permeabilized in 0.5 % Tween-20 before 1:100 antibody incubation in staining buffer (PBS, pH7.2, 0.5 % BSA, 2mM EDTA, 0.1 % Tween-20).
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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