Ezrin Antibody (3C12)

Novus Biologicals | Catalog # NB600-1310

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3C12
View all Ezrin Primary Antibodies »

Product Specifications

Immunogen

BALB/C mice were immunized with a recombinant fragment of human ezrin corresponding to amino acid 362-585.

Localization

Cell membrane

Specificity

This antibody reacts with a protein of 81 kDa. Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. Ezrin is a cytoplasmic protein, enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Ezrin Antibody (3C12)

Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (3C12) [NB600-1310]

Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (3C12) [NB600-1310]

Immunocytochemistry/Immunofluorescence: Ezrin Antibody (3C12) [NB600-1310] - Cultured Rat hippocampal neurons, courtesy of customer.
Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310]

Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310]

Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310] - Formalin fixed paraffin embedded lung adenocarcinoma stained with Ezrin antibody (NB600-1310).
Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310]

Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310]

Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310] - Formalin fixed paraffin embedded human lung stained with Ezrin antibody.

Applications for Ezrin Antibody (3C12)

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:100-1:750
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Reviewed Applications

Read 1 review rated 4 using NB600-1310 in the following applications:

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Ezrin

Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. It is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity and is expressed in epithelial cells but not in mesenchymal cells. It is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.

Alternate Names

CVIL, CVL, Cytovillin 2, EZR, p81, VIL2, Villin 2

Entrez Gene IDs

7430 (Human); 22350 (Mouse); 54319 (Rat)

Gene Symbol

EZR

Additional Ezrin Products

Product Documents for Ezrin Antibody (3C12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ezrin Antibody (3C12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Ezrin Antibody (3C12) (1)

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  • Ezrin Antibody (3C12)
    Name: Janosch Heller
    Application: Immunocytochemistry
    Sample Tested: Cultured rat hippocampal neurons
    Species: Rat
    Verified Customer | Posted 03/27/2017
    Fixation: 3% formaldehyde (TAAB) in PBS, 15 minutes RT Blocking: 10% donkey serum in PBS, 1 hour RT Permeabilization: 0.2% Triton-X100 Primary Antibody: 1:100 in 5% horse serum in PBS with 0.2% Triton, overnight, 4°C Wash: three times wash with PBS with 0.2% Triton, 10 min each Secondary Antibody: Invitrogen goat anti-mouse Alexa568, 1:500 in PBS with 0.2% Triton, 2 hours, RT Wash: three times wash with PBS, 10 min each Microscope: Confocal (Zeiss LSM710) The antibody labels actin filaments in filopodia.
    Ezrin Antibody (3C12) NB600-1310

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