Ezrin Antibody (6F1C3) - BSA Free

Novus Biologicals | Catalog # NBP2-52464

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6F1C3

Format

BSA Free
View all Ezrin Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Ezrin (AA: 292-464) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

69.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ezrin Antibody (6F1C3) - BSA Free

Western Blot: Ezrin Antibody (6F1C3)BSA Free [NBP2-52464]

Western Blot: Ezrin Antibody (6F1C3)BSA Free [NBP2-52464]

Western Blot: Ezrin Antibody (6F1C3) [NBP2-52464] - Analysis using EZR mouse mAb against Jurkat (1), A431 (2), MCF-7 (3) and Hela (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (6F1C3) - BSA Free [NBP2-52464]

Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (6F1C3) - BSA Free [NBP2-52464]

Immunocytochemistry/Immunofluorescence: Ezrin Antibody (6F1C3) [NBP2-52464] - Immunofluorescence analysis of Hela cells using EZR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow Cytometry: Ezrin Antibody (6F1C3) - BSA Free [NBP2-52464]

Flow Cytometry: Ezrin Antibody (6F1C3) - BSA Free [NBP2-52464]

Flow Cytometry: Ezrin Antibody (6F1C3) [NBP2-52464] - Analysis of A431 cells using EZR mouse mAb (green) and negative control (red).
Western Blot: Ezrin Antibody (6F1C3)BSA Free [NBP2-52464]

Western Blot: Ezrin Antibody (6F1C3)BSA Free [NBP2-52464]

Western Blot: Ezrin Antibody (6F1C3) [NBP2-52464] - Analysis using EZR mAb against HEK293 (1) and EZR (AA: 292-464)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Ezrin Antibody (6F1C3)BSA Free [NBP2-52464]

Western Blot: Ezrin Antibody (6F1C3)BSA Free [NBP2-52464]

Western Blot: Ezrin Antibody (6F1C3) [NBP2-52464] - Analysis using EZR mAb against human EZR (AA: 292-464) recombinant protein. (Expected MW is 47.1 kDa)
ELISA: Ezrin Antibody (6F1C3) - BSA Free [NBP2-52464]

ELISA: Ezrin Antibody (6F1C3) - BSA Free [NBP2-52464]

ELISA: Ezrin Antibody (6F1C3) [NBP2-52464] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for Ezrin Antibody (6F1C3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ezrin

Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. It is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity and is expressed in epithelial cells but not in mesenchymal cells. It is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.

Alternate Names

CVIL, CVL, Cytovillin 2, EZR, p81, VIL2, Villin 2

Gene Symbol

EZR

Additional Ezrin Products

Product Documents for Ezrin Antibody (6F1C3) - BSA Free

Certificate of Analysis

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Product Specific Notices for Ezrin Antibody (6F1C3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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