Ezrin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16396

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Human, Rat

Predicted:

Bovine (96%), Canine (98%), Chicken (92%), Chimpanzee (99%), Rabbit (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Ezrin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ezrin. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (85%).

Localization

Apical cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection; microvillus membrane; ruffle membrane; Cytoplasm; cell cortex

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Ezrin Antibody - BSA Free (NBP2-16396) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Ezrin Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Ezrin Antibody - BSA Free

Western Blot: Ezrin Antibody [NBP2-16396]

Western Blot: Ezrin Antibody [NBP2-16396]

Western Blot: Ezrin Antibody [NBP2-16396] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody [NBP2-16396]

Immunocytochemistry/ Immunofluorescence: Ezrin Antibody [NBP2-16396]

Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP2-16396] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ezrin stained by Ezrin antibody [N2C2], Internal diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396]

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396]

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - Rat duodenum. Ezrin antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396]

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396]

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - C2C12 xenograft, using Ezrin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396]

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396]

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - Human colon carcinoma, using Ezrin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Ezrin Antibody

IHC-Whole-Mount- Ezrin Antibody [NBP2-16396]-

IHC-Whole-Mount- Ezrin Antibody [NBP2-16396]--Fixed zebrafish embryos. Ezrin antibody [N2C2], Internal dilution: 1:200.
Ezrin Antibody

Western Blot: Ezrin Antibody [NBP2-16396] -

Western Blot: Ezrin Antibody [NBP2-16396] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Ezrin Antibody - BSA Free

Western Blot: Ezrin Antibody [NBP2-16396] -

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal (NBP2-16396) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Ezrin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Ezrin Antibody [NBP2-16396] -

Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: Ezrin protein stained by Ezrin antibody [N2C2], Internal (NBP2-16396) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Ezrin Antibody - BSA Free

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] -

Ezrin antibody [N2C2], Internal detects Ezrin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
Ezrin stained by Ezrin antibody [N2C2], Internal (NBP2-16396) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Ezrin Antibody - BSA Free

Immunohistochemistry: Ezrin Antibody [NBP2-16396] -

Ezrin antibody [N2C2], Internal detects Ezrin protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Ezrin stained by Ezrin antibody [N2C2], Internal (NBP2-16396) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Applications for Ezrin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry Whole-Mount

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000

Reviewed Applications

Read 1 review rated 5 using NBP2-16396 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ezrin

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq]

Alternate Names

CVIL, CVL, Cytovillin 2, EZR, p81, VIL2, Villin 2

Gene Symbol

EZR

UniProt

P15311 (Human)

Additional Ezrin Products

Product Documents for Ezrin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Ezrin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Ezrin Antibody - BSA Free

Customer Reviews for Ezrin Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Hela whole cell lysate
    Species: Human
    Verified Customer | Posted 10/16/2015
    Hela cells lysate 25 ug protein loaded on SDS gel (4-12%) used Bis-Tris gel for electrophoresis. Major bands ~69 kDa
    Ezrin Antibody - BSA Free NBP2-16396

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Protocols

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