Q: Why is HEPES used in cell culture?

A: HEPES (N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid, pKa@37oC=7.3, useful range pH 6.6 – 8.0) is one of several biological buffers that can be used to stabilize the pH of cell culture medium. As cells grow and metabolize the cell culture medium nutrients, they typically produce excess acid – that lowers the pH of the medium. This lowering of pH can be detrimental to the continued growth of cells in culture. HEPES provides more “buffering” capacity than the bicarbonate normally found in cell culture medium – thus slowing the decline in pH as cells metabolize the nutrient medium. For some cell lines this extends the time between “feedings” and promotes healthier cell growth. HEPES can show toxicity with some cell lines, so it is not a universal panacea. Other useful biological buffers include MOPS (pKa@37oC=7.0), TRIS (pKa@37oC=7.8), and PIPES (pKa@37oC=6.7).

F-12, 25 mM HEPES, GlutaminePlus, no phenol red

R&D Systems | Catalog # M15650

Submit a review

Product Documents

This item is in process of being discontinued.

Discontinued Product

M15650 has been discontinued. View all Cell Culture Media products.

Product Summary
Product Specifications
Preparation & Storage
Product Documents

Key Product Details

Features

Ham’s Nutrient Mixture F-12 with 25 mM HEPES buffer and GlutaminePlus. Without phenol red.

Applications

Cell Culture

Product Summary for F-12, 25 mM HEPES, GlutaminePlus, no phenol red

What is Ham's Nutrient Mixture F-12 Medium?

Ham’s Nutrient Mixtures, originally designed to support the serum-free growth of Chinese Hamster Ovary (CHO) cells, are now used with serum supplementation for a variety of cell types. Ham’s Nutrient Mixture F-12 features higher concentrations of some components, as compared to Ham’s F-10. Ham’s F-12 includes including versions with HEPES buffer for a more effective buffering capacity.

Each lot of F-12 is prepared from powdered base medium, tissue culture-grade water, and is sterile filtered using a 0.2 micron filter. Representative samples of each lot of F-12 are tested to confirm the absence of bacterial or fungal contamination using methods adapted from the current U.S. Pharmacopeia. F-12 is manufactured in our ISO 9001:2015 certified facility.

For the specific media formulation, please refer to the datasheet.

Product Specifications

Sterility Testing

Pass

Formulation, Preparation, and Storage

Purification

0.2 μm filtered

Shipping

Ambient

Storage

2 - 8 °C, protected from light

Product Documents for F-12, 25 mM HEPES, GlutaminePlus, no phenol red

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Customer Reviews for F-12, 25 mM HEPES, GlutaminePlus, no phenol red

There are currently no reviews for this product. Be the first to review F-12, 25 mM HEPES, GlutaminePlus, no phenol red and earn rewards!

Have you used F-12, 25 mM HEPES, GlutaminePlus, no phenol red?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image