F-12, 25 mM HEPES, no glutamine

Atlanta Biologicals media and balanced salt solutions are now sold under the R&D Systems brand
Catalog # Availability Size / Price Qty
F-12, 25 mM HEPES, no glutamine
1 Image
Product Details

F-12, 25 mM HEPES, no glutamine Summary

Ham’s Nutrient Mixture F-12 with 25 mM HEPES buffer. Without L-glutamine.

What is Ham's Nutrient Mixture F-12 Medium?

Ham’s Nutrient Mixtures, originally designed to support the serum-free growth of Chinese Hamster Ovary (CHO) cells, are now used with serum supplementation for a variety of cell types. Ham’s Nutrient Mixture F-12 features higher concentrations of some components, as compared to Ham’s F-10. Ham’s F-12 includes including versions with HEPES buffer for a more effective buffering capacity.

Each lot of F-12 is prepared from a powdered base medium and tissue culture-grade water. Representative samples of each lot of F-12 are tested to confirm the absence of bacterial or fungal contamination using methods adapted from the current U.S. Pharmacopeia. F-12 is manufactured in our ISO 9001:2015 certified facility.

For the specific media formulation, please refer to the datasheet.


Sterility Testing
Shipping Conditions
2 - 8 °C, protected from light
Cell Culture
Country of Origin


For research use only. Not for diagnositic use.

Product Datasheets

Data Example

F-12, 25 mM HEPES, no glutamine

Reagents Provided


No product specific FAQs exist for this product, however you may

View all FAQs

Reviews for F-12, 25 mM HEPES, no glutamine

There are currently no reviews for this product. Be the first to review F-12, 25 mM HEPES, no glutamine and earn rewards!

Have you used F-12, 25 mM HEPES, no glutamine?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review