F-12, 25 mM HEPES, no glutamine

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F-12, 25 mM HEPES, no glutamine
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F-12, 25 mM HEPES, no glutamine Summary

Ham’s Nutrient Mixture F-12 with 25 mM HEPES buffer. Without L-glutamine.

What is Ham's Nutrient Mixture F-12 Medium?

Ham’s Nutrient Mixtures, originally designed to support the serum-free growth of Chinese Hamster Ovary (CHO) cells, are now used with serum supplementation for a variety of cell types. Ham’s Nutrient Mixture F-12 features higher concentrations of some components, as compared to Ham’s F-10. Ham’s F-12 includes including versions with HEPES buffer for a more effective buffering capacity.

Each lot of F-12 is prepared from powdered base medium, tissue culture-grade water, and is sterile filtered using a 0.2 micron filter. Representative samples of each lot of F-12 are tested to confirm the absence of bacterial or fungal contamination using methods adapted from the current U.S. Pharmacopeia. F-12 is manufactured in our ISO 9001:2015 certified facility.

For the specific media formulation, please refer to the datasheet.


0.2 μm filtered
Sterility Testing
Shipping Conditions
2 - 8 °C, protected from light
Cell Culture
Country of Origin


For research use only. Not for diagnostic use.

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