Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2E3

Format

Azide and BSA Free
View all FA2H Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human FA2H(NP_077282) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FA2H Antibody (OTI2E3) - Azide and BSA Free

Western Blot: FA2H Antibody (OTI2E3)Azide and BSA Free [NBP2-72192]

Western Blot: FA2H Antibody (OTI2E3)Azide and BSA Free [NBP2-72192]

Western Blot: FA2H Antibody (OTI2E3) - Azide and BSA Free [NBP2-72192] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FA2H.
Immunocytochemistry/ Immunofluorescence: FA2H Antibody (OTI2E3) - Azide and BSA Free [NBP2-72192]

Immunocytochemistry/ Immunofluorescence: FA2H Antibody (OTI2E3) - Azide and BSA Free [NBP2-72192]

Immunocytochemistry/Immunofluorescence: FA2H Antibody (OTI2E3) - Azide and BSA Free [NBP2-72192] - Analysis of COS7 cells transiently transfected with pCMV6-ENTRY FA2H.

Applications for FA2H Antibody (OTI2E3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FA2H

Sphingolipids are a large class of lipids found in all eukaryotic cells and are involved in numerous cellular processes. The structural diversity of sphingolipids stems from more than 300 distinct head groups, as well as from modifications of the hydrophobic ceramide moiety. FA2H catalyzes a common modification of the ceramide moiety: hydroxylation at the 2 position of the N-acyl chain. Sphingolipids containing 2-hydroxy fatty acid are common in nervous and epidermal tissue. Glycosphingolipids containing a high proportion of 2-hydroxy fatty acid are critical components of myelin, and several very long chain ceramides with 2-hydroxy fatty acids are important for the permeability barrier function of epidermis (Alderson et al., 2004 [PubMed 15337768]).[supplied by OMIM]

Alternate Names

FAAHFAXDC1, FAH1, fatty acid 2-hydroxylase, Fatty acid alpha-hydroxylase, fatty acid hydroxylase domain containing 1, FLJ25287, SCS7, spastic paraplegia 35 (autosomal recessive), SPG35

Gene Symbol

FA2H

Additional FA2H Products

Product Documents for FA2H Antibody (OTI2E3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for FA2H Antibody (OTI2E3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FA2H Antibody (OTI2E3) - Azide and BSA Free

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Protocols

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FAQs

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