FABP1/L-FABP Antibody (8Q8S10)
Novus Biologicals | Catalog # NBP3-15353
Recombinant Monoclonal Antibody
Loading...
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8Q8S10 expressed in HEK293
Loading...
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FABP1/L-FABP (P07148). MSFSGKYQLQSQENFEAFMKAIGLPEELIQKGKDIKGVSEIVQNGKHFKFTITAGSKVIQNEFTVGEECELETMTGEKVKTVVQLEGDNKLVTTFKNIKS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FABP1/L-FABP Antibody (8Q8S10)
Western Blot: FABP1/L-FABP Antibody (8Q8S10) [NBP3-15353]
Western Blot: FABP1/L-FABP Antibody (8Q8S10) [NBP3-15353] - Western blot analysis of extracts of various cell lines, using FABP1/L-FABP Rabbit mAb (NBP3-15353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.Immunocytochemistry/ Immunofluorescence: FABP1/L-FABP Antibody (8Q8S10) [NBP3-15353]
Immunocytochemistry/Immunofluorescence: FABP1/L-FABP Antibody (8Q8S10) [NBP3-15353] - Immunofluorescence analysis of U-2 OS cells using FABP1/L-FABP Rabbit mAb (NBP3-15353) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunocytochemistry/ Immunofluorescence: FABP1/L-FABP Antibody (8Q8S10) [NBP3-15353]
Immunocytochemistry/Immunofluorescence: FABP1/L-FABP Antibody (8Q8S10) [NBP3-15353] - Immunofluorescence analysis of NIH-3T3 cells using FABP1/L-FABP Rabbit mAb (NBP3-15353) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Western Blot: FABP1/L-FABP Antibody (8Q8S10) [FABP1/L-FABP] -
Western Blot: FABP1/L-FABP Antibody (8Q8S10) [FABP1/L-FABP] - Western blot analysis of various lysates using FABP1/L-FABP Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western Blot: FABP1/L-FABP Antibody (8Q8S10) [FABP1/L-FABP] -
Western Blot: FABP1/L-FABP Antibody (8Q8S10) [FABP1/L-FABP] - Western blot analysis of lysates from Hep G2 cells using FABP1/L-FABP Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Applications for FABP1/L-FABP Antibody (8Q8S10)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FABP1/L-FABP
Long Name
Fatty Acid-Binding Protein 1
Alternate Names
L-FABP, LFABP
Gene Symbol
FABP1
Additional FABP1/L-FABP Products
Product Documents for FABP1/L-FABP Antibody (8Q8S10)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FABP1/L-FABP Antibody (8Q8S10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for FABP1/L-FABP Antibody (8Q8S10)
There are currently no reviews for this product. Be the first to review FABP1/L-FABP Antibody (8Q8S10) and earn rewards!
Have you used FABP1/L-FABP Antibody (8Q8S10)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...