Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # FABP5/3750
View all FABP5/E-FABP Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human FABP5/E-FABP protein (Uniprot: Q01469)

Reactivity Notes

Others not known.

Localization

Nuclear, Cytoplasmic

Marker

Marker of Metastatic Potential in Colorectal Cancer

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

15.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79929)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for FABP5/E-FABP Antibody (FABP5/3750)

Western Blot: FABP5/E-FABP Antibody (FABP5/3750) [NBP2-79795]

Western Blot: FABP5/E-FABP Antibody (FABP5/3750) [NBP2-79795]

Western Blot: FABP5/E-FABP Antibody (FABP5/3750) [NBP2-79795] - Western Blot Analysis of HeLa cell lysate using FABP5/E-FABP Antibody (FABP5/3750).
Immunohistochemistry-Paraffin: FABP5/E-FABP Antibody (FABP5/3750) [NBP2-79795]

Immunohistochemistry-Paraffin: FABP5/E-FABP Antibody (FABP5/3750) [NBP2-79795]

Immunohistochemistry-Paraffin: FABP5/E-FABP Antibody (FABP5/3750) [NBP2-79795] - Formalin-fixed, paraffin-embedded human Liver tissue stained with FABP5/E-FABP Antibody (FABP5/3750).
Protein Array: FABP5/E-FABP Antibody (FABP5/3750) [NBP2-79795]

Protein Array: FABP5/E-FABP Antibody (FABP5/3750) [NBP2-79795]

Protein Array: FABP5/E-FABP Antibody (FABP5/3750) [NBP2-79795] - Analysis of Protein Array containing more than 19,000 full-length human proteins using FABP5/E-FABP Antibody (FABP5/3750). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5

Applications for FABP5/E-FABP Antibody (FABP5/3750)

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

0.1-0.2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistology (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: FABP5/E-FABP

Fatty Acid Binding Protein 5 encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.

Long Name

Fatty Acid-Binding Protein 5

Alternate Names

E-FABP, EFABP

Gene Symbol

FABP5

UniProt

Q01469

Additional FABP5/E-FABP Products

Product Documents for FABP5/E-FABP Antibody (FABP5/3750)

Certificate of Analysis

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Product Specific Notices for FABP5/E-FABP Antibody (FABP5/3750)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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