FACL4 Antibody (GT186) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13533

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT186

Format

Azide and BSA Free
View all FACL4 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FACL4. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for FACL4 Antibody (GT186) - Azide and BSA Free

Western Blot: FACL4 Antibody (GT186) [NBP3-13533]

Western Blot: FACL4 Antibody (GT186) [NBP3-13533]

Western Blot: FACL4 Antibody (GT186) [NBP3-13533] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [GT186] (NBP3-13533) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: FACL4 Antibody (GT186) [NBP3-13533]

Immunohistochemistry-Paraffin: FACL4 Antibody (GT186) [NBP3-13533]

Immunohistochemistry-Paraffin: FACL4 Antibody (GT186) [NBP3-13533] - FACL4 antibody [GT186] detects FACL4 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. FACL4 stained by FACL4 antibody [GT186] (NBP3-13533) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: FACL4 Antibody (GT186) [NBP3-13533]

Western Blot: FACL4 Antibody (GT186) [NBP3-13533]

Western Blot: FACL4 Antibody (GT186) [NBP3-13533] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [GT186] (NBP3-13533) diluted at 1:500.
FACL4 Antibody (GT186)

Western Blot: FACL4 Antibody (GT186) [NBP3-13533] -

Western Blot: FACL4 Antibody (GT186) [NBP3-13533] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [GT186] (NBP3-13533) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
FACL4 Antibody (GT186) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: FACL4 Antibody (GT186) [NBP3-13533] -

FACL4 antibody [GT186] detects FACL4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: FACL4 stained by FACL4 antibody [GT186] (NBP3-13533) diluted at 1:100.
Blue: Hoechst 33342 staining.

Applications for FACL4 Antibody (GT186) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FACL4

The protein encoded by the FACL4 gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Althoughdiffering in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this familyconvert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis andfatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme maycontribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcriptvariants. (provided by RefSeq)

Alternate Names

ACS4mental retardation, X-linked 68, acyl-CoA synthetase 4, acyl-CoA synthetase long-chain family member 4, EC 6.2.1.3, FACL4long-chain 4, LACS 4, LACS4MRX68, lignoceroyl-CoA synthase, Long-chain acyl-CoA synthetase 4, long-chain fatty-acid-Coenzyme A ligase 4, long-chain-fatty-acid--CoA ligase 4, mental retardation, X-linked 63, MRX63

Gene Symbol

ACSL4

Additional FACL4 Products

Product Documents for FACL4 Antibody (GT186) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for FACL4 Antibody (GT186) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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