FANCG Antibody

Novus Biologicals | Catalog # NBP1-06035

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FANCG Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence LEEFRTSLPKSCDL corresponding to C-Terminus according to NP_004620.1.

Epitope

LEEFRTSLPKSCDL

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FANCG Antibody

Western Blot: FANCG Antibody [NBP1-06035]

Western Blot: FANCG Antibody [NBP1-06035]

Western Blot: FANCG Antibody [NBP1-06035] - Staining of Jurkat nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: FANCG Antibody [NBP1-06035]

Immunohistochemistry-Paraffin: FANCG Antibody [NBP1-06035]

Immunohistochemistry-Paraffin: FANCG Antibody [NBP1-06035] - staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: FANCG Antibody [NBP1-06035]

Western Blot: FANCG Antibody [NBP1-06035]

Western Blot: FANCG Antibody [NBP1-06035] - Analysis of FANCG using this antibody at 0.5 ug/ml in HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: FANCG Antibody [NBP1-06035]

Western Blot: FANCG Antibody [NBP1-06035]

Western Blot: FANCG Antibody [NBP1-06035] - HEK293 overexpressing FANCG and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: FANCG Antibody [NBP1-06035]

Immunohistochemistry-Paraffin: FANCG Antibody [NBP1-06035]

Immunohistochemistry-Paraffin: FANCG Antibody [NBP1-06035] - staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for FANCG Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 1 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in HeLa lysates (calculated MW of 68.6 kDa band according to NP_004620.1). In transfected HEK293 transiently expressing FANCG a band of approx. 65 kDa is observed. This band is not observed in the non-transfected HEK293.
IHC: Paraffin embedded Human Uterus and Spleen.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FANCG

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G. [provided by RefSeq]

Long Name

Fanconi Anemia Complementation Group G

Alternate Names

XRCC9

Entrez Gene IDs

2189 (Human)

Gene Symbol

FANCG

UniProt

NP_004620.1 (Human)

Additional FANCG Products

Product Documents for FANCG Antibody

Certificate of Analysis

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Product Specific Notices for FANCG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

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Protocols

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