Fascin 2 Antibody

Novus Biologicals | Catalog # NBP1-06036

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Predicted:

Canine (100%), Human (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence CHHRGSNQLDTNR corresponding to internal region according to NP_036550.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_001070650.1 and NP_036550.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Fascin 2 Antibody

Immunohistochemistry-Paraffin: Fascin 2 Antibody [NBP1-06036]

Immunohistochemistry-Paraffin: Fascin 2 Antibody [NBP1-06036]

Immunohistochemistry-Paraffin: Fascin 2 Antibody [NBP1-06036] - Staining of paraffin embedded Mouse Brain. Antibody at 4 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for Fascin 2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

4 ug/mL

Peptide ELISA

Detection limit 1:64000
Application Notes
WB: Preliminary experiments gave bands at approx. 75 kDa band and 22 kDa band in mouse eye lysates after 0.05 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 57.4 kDa band according to NP_001070650.1 and 55.1 kDa band according to NP_036550.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P successful usage on sections of mouse cochlea is reported in scientific literature (PMID: 20660251).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Fascin 2

The Fascin 2 gene encodes a member of the fascin protein family. Fascins crosslink actin into filamentous bundles within dynamic cell extensions. This family member is proposed to play a role in photoreceptor disk morphogenesis. A mutation in this gene results in

Alternate Names

fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotuspurpuratus), fascin-2, Retinal fascin, retinal), RFSNRP30fascin (Strongylocentrotus purpuratus) homolog 2 (actin-bundling protein

Entrez Gene IDs

25794 (Human); 238021 (Mouse); 303741 (Rat)

Gene Symbol

FSCN2

UniProt

NP_001070650.1 (Human)

Additional Fascin 2 Products

Product Documents for Fascin 2 Antibody

Certificate of Analysis

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Product Specific Notices for Fascin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fascin 2 Antibody

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Protocols

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