Fatty Acid Synthase/FASN Antibody (325) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43822

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 325

Format

Azide and BSA Free
View all Fatty Acid Synthase/FASN Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Fatty Acid Synthase/FASN. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

273 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fatty Acid Synthase/FASN Antibody (325) - Azide and BSA Free

Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822]

Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822]

Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug BCL-1 whole cell lysate/extract 5 % SDS-PAGE Fatty Acid Synthase antibody dilution: 1:500
Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822]

Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822]

Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE Fatty Acid Synthase antibody dilution: 1:5000.
Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822]

Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822]

Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [GT325].
Fatty Acid Synthase/FASN Antibody (325)

Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] -

Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase/FASN antibody [GT325] (NBP2-43822) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)
Fatty Acid Synthase/FASN Antibody (325) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] -

Fatty Acid Synthase antibody [GT325] detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Fatty Acid Synthase stained by Fatty Acid Synthase antibody [GT325] (NBP2-43822) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar= 10um.

Applications for Fatty Acid Synthase/FASN Antibody (325) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Fatty Acid Synthase/FASN

Fatty Acid Synthase (FAS) is a very large enzymatic system involved in de novo lipogenesis (triglyceride production). Specifically, FAS catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. It is also one of the accepted markers for insulin resistance and SREBP, LXR activation.

In some cancer cell lines, FAS has been found to be fused with ER-alpha. Therefore, FAS antibodies are useful tools for studies on fatty acid synthesis and research on certian cancers.

Alternate Names

FASN, OA-519, SDR27X1

Gene Symbol

FASN

Additional Fatty Acid Synthase/FASN Products

Product Documents for Fatty Acid Synthase/FASN Antibody (325) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Fatty Acid Synthase/FASN Antibody (325) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fatty Acid Synthase/FASN Antibody (325) - Azide and BSA Free

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Protocols

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