FBPase 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84721

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FBPase 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYAKDFDPAVTEYIQRKKFPPDNSAP

Reactivity Notes

Reactivity reported in scientific literature (PMID: 25043030). Rat (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FBPase 1 Antibody - BSA Free

Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721]

Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721]

Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721] - Staining in human liver and skeletal muscle tissues. Corresponding FBP1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721]

Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721]

Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721]

Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721]

Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721]

Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721]

Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721]

Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721]

Immunohistochemistry-Paraffin: FBPase 1 Antibody [NBP1-84721] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Simple Western: FBPase 1 Antibody [NBP1-84721]

Simple Western: FBPase 1 Antibody [NBP1-84721]

Simple Western: FBPase 1 Antibody [NBP1-84721] - Electropherogram image(s) of corresponding Simple Western lane view. FBPase 1 antibody was used at 1:20 dilution on RT-4 and NIH-3T3 lysates(s).
FBPase 1 Antibody - BSA Free Western Blot: FBPase 1 Antibody - BSA Free [NBP1-84721]

Western Blot: FBPase 1 Antibody - BSA Free [NBP1-84721]

Analysis in human liver tissue.

Applications for FBPase 1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and NIH-3T3, separated by Size, antibody dilution of 1:20, apparent MW was 41 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FBPase 1

Fructose-1,6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. [provided by RefSeq]

Long Name

Fructose-1,6-bisphosphatase 1

Alternate Names

FBP1

Gene Symbol

FBP1

Additional FBPase 1 Products

Product Documents for FBPase 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FBPase 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FBPase 1 Antibody - BSA Free

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Protocols

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