FBXO44 Antibody

Novus Biologicals | Catalog # NBP1-51915

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FBXO44 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-AALTPPEPPSAEP corresponding to C-Terminus according to NP_904319.1.

Epitope

C-AALTPPEPPSAEP

Specificity

This antibody is expected to recognize isoform 2 (NP_904319.1 and NP_9043201

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FBXO44 Antibody

Immunocytochemistry/ Immunofluorescence: FBXO44 Antibody [NBP1-51915]

Immunocytochemistry/ Immunofluorescence: FBXO44 Antibody [NBP1-51915]

Immunocytochemistry/Immunofluorescence: FBXO44 Antibody [NBP1-51915] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: FBXO44 Antibody [NBP1-51915]

Flow Cytometry: FBXO44 Antibody [NBP1-51915]

Flow Cytometry: FBXO44 Antibody [NBP1-51915] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: FBXO44 Antibody [NBP1-51915]

Immunocytochemistry/ Immunofluorescence: FBXO44 Antibody [NBP1-51915]

Immunocytochemistry/Immunofluorescence: FBXO44 Antibody [NBP1-51915] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for FBXO44 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:8000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FBXO44

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It is also a member of the NFB42 (neural F Box 42 kDa) family, similar to F-box only protein 2 and F-box only protein 6. Four alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene.

Alternate Names

FBG3 DKFZp781J0852, F-box gene 3, F-box only protein 44, F-box protein 44, F-box protein FBX30, F-box/G-domain protein 3, FBX30FBX6AFbx44, FBX44, Fbxo6a, MGC14140

Entrez Gene IDs

93611 (Human)

Gene Symbol

FBXO44

UniProt

NP_904319.1 (Human)

Additional FBXO44 Products

Product Documents for FBXO44 Antibody

Certificate of Analysis

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Product Specific Notices for FBXO44 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FBXO44 Antibody

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Protocols

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