FBXW7/Cdc4 Antibody

Novus Biologicals | Catalog # NBP2-89145

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all FBXW7/Cdc4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within human FBXW7/Cdc4 aa 84-327. (SwissProt: Q969H0 Human; SwissProt: Q8VBV4 Mouse; SwissProt: D3Z902 Rat)

Localization

Cytoplasm. Nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FBXW7/Cdc4 Antibody

Western Blot: FBXW7/Cdc4 Antibody [NBP2-89145]

Western Blot: FBXW7/Cdc4 Antibody [NBP2-89145]

Western Blot: FBXW7/Cdc4 Antibody [NBP2-89145] - Analysis of FBXW7 on different lysates using anti-FBXW7 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse spleen tissue Lane 2: Rat lung tissue Lane 3: HepG2
Immunocytochemistry/ Immunofluorescence: FBXW7/Cdc4 Antibody [NBP2-89145]

Immunocytochemistry/ Immunofluorescence: FBXW7/Cdc4 Antibody [NBP2-89145]

Immunocytochemistry/Immunofluorescence: FBXW7/Cdc4 Antibody [NBP2-89145] - Staining FBXW7 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow Cytometry: FBXW7/Cdc4 Antibody [NBP2-89145]

Flow Cytometry: FBXW7/Cdc4 Antibody [NBP2-89145]

Flow Cytometry: FBXW7/Cdc4 Antibody [NBP2-89145] - Analysis of SH-SY5Y cells with FBXW7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence: FBXW7/Cdc4 Antibody [NBP2-89145]

Immunocytochemistry/ Immunofluorescence: FBXW7/Cdc4 Antibody [NBP2-89145]

Immunocytochemistry/Immunofluorescence: FBXW7/Cdc4 Antibody [NBP2-89145] - Staining FBXW7 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications for FBXW7/Cdc4 Antibody

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:500-1:2000

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH7.4), 0.2% BSA, 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FBXW7/Cdc4

Auxin-inducible degron (AID) technology allows rapid depletion of proteins in animal cells and fungi. Auxin includes chemicals such as IAA (indole-3-aceticacid) and NAA (1-naphtalene acetic acid) and includes inhibitors in plants. For the degradation, and SCF ubiquitin ligase complex containing the TIR1 protein is activated via the association of auxin with TIR1. Because the core SCF components are conserved in all eukaryotic cells, it is possible to transplant the degradation pathway to yeast and mammalian cells by expressing TIR1. In those cells expressing TIR1, a protein fused with degron (AID degron) derived from AUX/IAA can be rapidly degraded in the presence of auxin. Anti-mini-AID tag can detected both mini-AID-tag and full-length AID-tag.

Long Name

F-box and WD Repeat Domain Containing 7

Alternate Names

AGO, Archipelago Homolog, Cdc4, FBW6, Fbw7, FBX30, FBXO30, FBXW6, SCF(Fbw7), SEL10

Gene Symbol

FBXW7

Additional FBXW7/Cdc4 Products

Product Documents for FBXW7/Cdc4 Antibody

Certificate of Analysis

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Product Specific Notices for FBXW7/Cdc4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail