Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-08997

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # AT130-5

Format

Azide and BSA Free
View all Fc gamma RII/CD32 Primary Antibodies »

Product Specifications

Immunogen

CD32-rat CD4 domain 3/4 fusion protein.

Specificity

This antibody is specific for murine Fc gamma RII/CD32, which is a Fc gamma receptor that expresses on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. Fc gamma RII/CD32 binds antibody-antigen immune complexes and mediates adaptive immune responses.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free [NBP3-08997]

Immunocytochemistry/ Immunofluorescence: Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free [NBP3-08997]

Immunocytochemistry/Immunofluorescence: Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric [NBP3-08997] - Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of AT130-5 (NBP3-08997) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-08997, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.

Applications for Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

The antibody binds to CD32, a surface receptor protein with a low affinity for IgG antibodies.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fc gamma RII/CD32

CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in downmodulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.

Long Name

Fc gamma Receptor II

Alternate Names

FcgRII

Gene Symbol

FCGR2B

Additional Fc gamma RII/CD32 Products

Product Documents for Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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