Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25404

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Rhesus Macaque (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1948 expressed in HEK293

Format

Azide and BSA Free
View all Fc gamma RIIB/CD32b Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Fc gamma RIIB/CD32b. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free

Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free [NBP3-25404] -

Immunocytochemistry/Immunofluorescence: Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free [NBP3-25404] - CD32B antibody [HL1948] detects CD32B protein at cell membrane by immunofluorescent analysis. Sample: U937 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD32B stained by CD32B antibody [HL1948] (NBP3-25404) diluted at 1:1000. Blue: Fluoroshield with DAPI.
Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free [NBP3-25404] -

Immunocytochemistry/Immunofluorescence: Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free [NBP3-25404] - CD32B antibody [HL1948] detects CD32B protein by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD32B stained by CD32B antibody [HL1948] (NBP3-25404) diluted at 1:500. Blue: Fluoroshield with DAPI.
Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free

Immunohistochemistry-Paraffin: Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free [NBP3-25404] -

Immunohistochemistry-Paraffin: Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free [NBP3-25404] - CD32B antibody [HL1948] detects CD32B protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. CD32B stained by CD32B antibody [HL1948] (NBP3-25404) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free

Western Blot: Fc gamma RIIB/CD32b Antibody (HL1948) [NBP3-25404] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD32B antibody [HL1948] (NBP3-25404) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fc gamma RIIB/CD32b

Low affinity IgG Fc receptor gamma II (Fc-gamma II), also known as CD32, is a member of the immunoreceptor tyrosine-based activating/inhibitory motif (ITAM/ITIM) family. Fc gamma receptors play a critical role in immunity by linking the IgG antibody mediated response with the regulatory functions of the immune system (1). Three classes of Fc gamma receptor exist; Fc gamma RI (CD64, a high affinity receptor), Fc gamma RII, and RIII (CD32 and CD16 respectively, both low affinity receptors). Fc gamma RIIa is an activating receptor, while RIIb1, RIIb2, and RIIb3 are inhibitory receptors generated by alternative splicing (1-2). Fc gamma RIIa is critical for antigen-presenting-cell activation and Fc gamma RIIb downregulates B-cell functions (3). Fc gamma RIIb has also been known to block the inflammatory immune response in mice (4). Once phosphorylated on tyrosine residues, Fc gamma RIIb associates with SHIP to downregulate phagocytosis.

Long Name

Fc gamma Receptor II

Alternate Names

CD32b, FCGR2B, FcgRIIB, FCRIIB

Gene Symbol

FCGR2B

Additional Fc gamma RIIB/CD32b Products

Product Documents for Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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