Fcgr4, also known as Fc Receptor-like 3 (Fcrl3) and CD16-2, is a recently described mouse protein that is closely related to human Fc gamma RIIIA (60% amino acid identity) and may be its true ortholog. Similar to other activating receptors, it possesses a charged residue within its transmembrane domain that is necessary for association with an ITAM bearing accessory protein. CD16-2 mRNA expression was observed in peripheral blood leukocytes, spleen, thymus, colon, and intestine.
FcgR4/CD16-2 Antibody (012) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-05890
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: FcgR4/CD16-2 Antibody (012) [NBP3-05890]](https://resources.rndsystems.com/images/products/FcgR4-CD16-2-Antibody-012-Immunocytochemistry-Immunofluorescence-NBP3-05890-img0001.jpg "Immunocytochemistry/ Immunofluorescence: FcgR4/CD16-2 Antibody (012) [NBP3-05890]")
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Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 012 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FcgR4/CD16-2 (Accession#: NP_653142.2; Met 1-Gln 203).
Reactivity Notes
Has cross-reactivity in ELISA with:
Human CD64 / FCGR1A
No cross-reactivity in ELISA with:
Human CD16a / FCGR3A / FcRIIIa
Human CD16b / FCGR3B / FcRIIIb
Human CD32a / FCGR2A
Human CD32b / FCGR2B
Mouse CD16 / FCGR3
Human CD64 / FCGR1A
No cross-reactivity in ELISA with:
Human CD16a / FCGR3A / FcRIIIa
Human CD16b / FCGR3B / FcRIIIb
Human CD32a / FCGR2A
Human CD32b / FCGR2B
Mouse CD16 / FCGR3
Specificity
Has cross-reactivity in ELISA with:
Human CD64 / FCGR1ANo cross-reactivity in ELISA with:
Human CD16a / FCGR3A / FcRIIIa
Human CD16b / FCGR3B / FcRIIIb
Human CD32a / FCGR2A
Human CD32b / FCGR2B
Mouse CD16 / FCGR3
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for FcgR4/CD16-2 Antibody (012) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: FcgR4/CD16-2 Antibody (012) [NBP3-05890]
Immunocytochemistry/Immunofluorescence: FcgR4/CD16-2 Antibody (012) [NBP3-05890] - Staining of Mouse FcGR4 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse FcGR4 monoclonal antibody (1:100) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.Applications for FcgR4/CD16-2 Antibody (012) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Flow Cytometry
1:25-1:100
Immunocytochemistry/ Immunofluorescence
1:50-1:1000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FcgR4/CD16-2
Long Name
Fc receptor, IgG, low affinity IV
Alternate Names
CD16-2, Fcrl3
Gene Symbol
Fcgr4
Additional FcgR4/CD16-2 Products
Product Documents for FcgR4/CD16-2 Antibody (012) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FcgR4/CD16-2 Antibody (012) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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