Fcgr4, also known as Fc Receptor-like 3 (Fcrl3) and CD16-2, is a recently described mouse protein that is closely related to human Fc gamma RIIIA (60% amino acid identity) and may be its true ortholog. Similar to other activating receptors, it possesses a charged residue within its transmembrane domain that is necessary for association with an ITAM bearing accessory protein. CD16-2 mRNA expression was observed in peripheral blood leukocytes, spleen, thymus, colon, and intestine.
FcgR4/CD16-2 Antibody (012) [FITC]
Novus Biologicals | Catalog # NBP3-05890F
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 012 expressed in HEK293
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Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FcgR4/CD16-2 (Accession#: NP_653142.2; Met 1-Gln 203).
Reactivity Notes
Has cross-reactivity in ELISA with:
Human CD64 / FCGR1A
No cross-reactivity in ELISA with:
Human CD16a / FCGR3A / FcRIIIa
Human CD16b / FCGR3B / FcRIIIb
Human CD32a / FCGR2A
Human CD32b / FCGR2B
Mouse CD16 / FCGR3
Human CD64 / FCGR1A
No cross-reactivity in ELISA with:
Human CD16a / FCGR3A / FcRIIIa
Human CD16b / FCGR3B / FcRIIIb
Human CD32a / FCGR2A
Human CD32b / FCGR2B
Mouse CD16 / FCGR3
Specificity
Has cross-reactivity in ELISA with:
Human CD64 / FCGR1ANo cross-reactivity in ELISA with:
Human CD16a / FCGR3A / FcRIIIa
Human CD16b / FCGR3B / FcRIIIb
Human CD32a / FCGR2A
Human CD32b / FCGR2B
Mouse CD16 / FCGR3
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Applications for FcgR4/CD16-2 Antibody (012) [FITC]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: FcgR4/CD16-2
Long Name
Fc receptor, IgG, low affinity IV
Alternate Names
CD16-2, Fcrl3
Gene Symbol
Fcgr4
Additional FcgR4/CD16-2 Products
Product Documents for FcgR4/CD16-2 Antibody (012) [FITC]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FcgR4/CD16-2 Antibody (012) [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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