Feline IL-8/CXCL8 Biotinylated Antibody

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Product Details
Supplemental Products

Feline IL-8/CXCL8 Biotinylated Antibody Summary

Species Reactivity
Detects feline IL-8/CXCL8 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human IL-8/CXCL8, recombinant canine IL-8/CXCL8 or recombinant porcine IL-8/CXCL8 is observed.  
Monoclonal Rat IgG2B Clone # 344610
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant feline IL-8/CXCL8
Accession # Q9XSX5.1
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration

Feline IL-8/CXCL8 Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Feline IL-8/CXCL8 Antibody (Catalog # MAB22772)

Standard: Recombinant Feline IL-8/CXCL8 Protein (Catalog # 2277-FL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-8/CXCL8

Interleukin 8 (IL-8), also named CXCL8, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil-activating protein 1 (NAP-1), neutrophil-activating factor (NAF) and granulocyte chemotactic peptide (GCP), belongs to the Glu-Leu-Arg motif containing (ELR+) CXC chemokine family and has been designated CXCL8. IL-8 is a potent neutrophil chemoattractant that recruits neutrophils to sites of inflammation. IL-8 also activates neutrophil functions and promotes angiogenesis. The biological activites of IL-8 is mediated by two types of G protein-coupled chemokine receptors, CXCR1 and CXCR2 (1, 2). In normal tissues, IL-8 expression and secretion is barely detectable. Upon stimulation by a wide range of pro-inflammatory signals including exposure to IL-1, TNF, bacterial or viral products, IL-8 production is rapidly induced in many different cell types (3, 4). Feline IL-8 encodes a 101 amino acid (aa) precursor protein with a putative 22 aa signal peptide. It shares 61% and 76% aa sequence identity with human and canine IL-8, respectively.

  1. Van Damme, J. et al. (1998) The Cytokine Handbook, A.W. Thomson, ed., Academic Press, NY p. 271.
  2. Heidemann, J. et al. (2003) J. Biol. Chem. 178:8508.
  3. Yang, M.P. et al. (2002) Vet. Immunol. Immunopathol. 86:43.
  4. Parhar, K. et al. (2003) Immunology 108:502.
Long Name
Interleukin 8
Entrez Gene IDs
3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names
3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

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