Ferritin Light Chain Antibody (X1) - Azide and BSA Free

Novus Biologicals | Catalog # H00002512-M16

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # X1

Format

Azide and BSA Free
View all Ferritin Light Chain Primary Antibodies »

Product Specifications

Immunogen

FTL (AAH04245, 1 a.a. ~ 175 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Ferritin Light Chain Antibody (X1) - Azide and BSA Free

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16]

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16]

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16] - Analysis of FTL expression in transfected 293T cell line by FTL monoclonal antibody (M16), clone X1. Lane 1: FTL transfected lysatE (20 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Ferritin Light Chain Antibody (X1) [H00002512-M16]

Immunocytochemistry/ Immunofluorescence: Ferritin Light Chain Antibody (X1) [H00002512-M16]

Immunocytochemistry/Immunofluorescence: Ferritin Light Chain Antibody (X1) [H00002512-M16] - Analysis of monoclonal antibody to FTL on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16]

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16]

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16] - Analysis of FTL expression in K-562 (Cat # L009V1).
Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16]

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16]

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16] - Analysis of FTL transfected lysate using anti-FTL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FTL rabbit polyclonal antibody.
Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16]

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16]

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16] - Analysis of FTL over-expressed 293 cell line, cotransfected with FTL Validated Chimera RNAi ( Cat # H00002512-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FTL monoclonal antibody (M16), clone X1 (Cat # H00002512-M16 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: Ferritin Light Chain Antibody (X1) [H00002512-M16] - Detection limit for recombinant GST tagged FTL is approximately 0.03ng/ml as a capture antibody.

Applications for Ferritin Light Chain Antibody (X1) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA, WB, IP and IF.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ferritin Light Chain

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

Alternate Names

Ferritin L subunit, ferritin L-chain, ferritin light chain, ferritin light polypeptide-like 3, ferritin, light polypeptide, MGC71996, NBIA3

Gene Symbol

FTL

UniProt

AAH04245 (Human)

Additional Ferritin Light Chain Products

Product Documents for Ferritin Light Chain Antibody (X1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Ferritin Light Chain Antibody (X1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Ferritin Light Chain Antibody (X1) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Ferritin Light Chain Antibody (X1) - Azide and BSA Free and earn rewards!

Have you used Ferritin Light Chain Antibody (X1) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies