Fetal Bovine Serum - Dialyzed 12-14kD, Heat Inactivated

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Fetal Bovine Serum - Dialyzed 12-14kD, Heat Inactivated
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Fetal Bovine Serum - Dialyzed 12-14kD, Heat Inactivated Summary

Dialyzed Fetal Bovine Serum is used in cell culture systems requiring a more defined environment of small molecules. Dialysis reduces the concentration of low molecular weight components such as nucleotides, amino acids, hormones, salts and various small proteins in serum.

Key Benefits

• Dialysis performed using a 12 - 14 kDa cutoff membrane
• Process controlled via glucose monitoring
• Monitored during dialysis to prevent peptide preciptation and inactivation in the FBS
• Excellent cell growth characteristics and low endotoxin
• USDA-Approved FBS of Mexico/Central American origin
• Manufactured in our ISO 9001:2015 certified facility
• Formerly sold under the Atlanta Biologicals brand

Why Use Dialyzed Fetal Bovine Serum, Heat Inactivated?

Pre-selected Fetal Bovine Serum, also known as Fetal Calf Serum (FCS), is dialyzed against physiological saline using a 12,000 to 14,000 Dalton cutoff membrane. The process is controlled by precisely monitoring the glucose concentration throughout the procedure. Exhaustive dialysis is not performed in order to prevent precipitation and inactivation of serum peptides. All lots of Dialyzed 12-14kD FBS are manufactured in our ISO 9001:2015 certified facility.

Heat Inactivation - Serum is inactivated by raising the temperature to 56 °C for 30 minutes under controlled conditions. The objective of heat inactivation is to destroy complement activity in the serum without affecting the growth-promoting characteristics of the product. Removal of complement activity from the serum is not required for most cell cultures, but may be necessary for cultures that are sensitive to the complement activity. Since heat inactivation of the serum may, to some extent, decrease the growth performance properties of the serum, this procedure should only be performed if actually required for optimal cell growth. Researchers should evaluate the applicability of heat inactivation in regards to their own application. If heat inactivation is required, the process should be carefully controlled to avoid increased formation of crystalline and flocculent precipitates, gelling of serum proteins and excessive loss of growth performance. Significant damage to serum can occur when it is subjected to higher than required temperatures or heated over extended lengths of time.

Protocol for Heat Inactivation of Serum Products.
Protocol for Fetal Bovine Serum Storage, Thawing, and Freezing.

Collection, Processing, and Testing of R&D Systems FBS

Collection and Processing - R&D Systems FBS is manufactured under a process that meets USDA requirements for animal products and ensures the product is traceable back to the date and location of collection. Our FBS manufacturing adheres to closed-system collection, processing in positive pressure, HEPA-filtered clean rooms, and triple 0.1 um filtration to remove contaminants. Strict control of the process from collection through processing enables us to provide a stable, consistent performing, and traceable supply of quality serum throughout global fluctuations in serum availability and regulatory requirements.

Virus Testing - Fetal Bovine Serum – Dialyzed 12-14kD undergoes testing for adventitious viruses, such as: Bovine Viral Diarrhea Virus (BVDV), Infectious Bovine Rhinotracheitis Virus (IBRV), Parainfluenza-3 Virus (PI-3V), Bluetongue Virus (BTV), Bovine Respiratory Syncytial Virus (BRSV), Bovine Parvovirus (BPV), Bovine Adenovirus, Type 3 (BAV-3), Bovine Adenovirus, Type 5 (BAV-5), Reo virus, and Rabies virus.

Biochemical Testing - R&D Systems FBS undergo biochemical profiling including total protein, albumin, globulin, IgG, total bilirubin, glucose, iron, cholesterol, triglycerides, sodium and potassium. View the product insert for a complete list of biochemical profiling components, which may vary between grades.

Chemical Analysis - Each lot of FBS is tested for osmolality, pH, hemoglobin, endotoxin (LAL method) and microbiological contamination (using methods recommended by the U.S. Pharmacopeia).

Specifications

Osmolality
260 -335 mOsm
Purification
Triple 0.1 μm filtered
Sterility Testing
Pass
Testing Additional
pH - 6.8-8.0

Endotoxin - ≤ 100.0 EU/mL by Limulus Amebocyte Lysate (LAL) Assay

Hemoglobin - 25 mg/dL
Shipping Conditions
On Dry Ice
Storage
-5 to -20 °C
Applications
Cell Culture
Species
Bovine

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Data Example

Fetal Bovine Serum - Dialyzed 12-14kD, Heat Inactivated

Reagents Provided

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