Fetal Bovine Serum - Embryonic Stem Cell Qualified, Heat Inactivated
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Fetal Bovine Serum - Embryonic Stem Cell Qualified, Heat Inactivated Summary
Why Use Embryonic Stem Cell Qualified Fetal Bovine Serum?
Only the highest quality Fetal Bovine Serum lots are selected for further testing regarding suitability for culturing embryonic stem cells. This screening includes plating efficiency, colony morphology, and cytotoxicity assays. ES Qualified FBS lots must offer outstanding performance with embryonic stem cells while maintaining the cells in their valuable undifferentiated state.
Why Heat Inactivate Serum?
The objective of heat inactivation is to destroy complement activity in the serum without affecting the growth-promoting characteristics of the product. Removal of complement activity from the serum is not required for most cell cultures, but may be necessary for cultures that are sensitive to the complement activity. Since heat inactivation of the serum may, to some extent, decrease the growth performance properties of the serum, this procedure should only be performed if actually required for optimal cell growth. Researchers should evaluate the applicability of heat inactivation in regards to their own application. If heat inactivation is required, the process should be carefully controlled to avoid increased formation of crystalline and flocculent precipitates, gelling of serum proteins and excessive loss of growth performance. Significant damage to serum can occur when it is subjected to higher than required temperatures or heated over extended lengths of time.
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