Fetal Bovine Serum - Hybridoma Qualified, Heat Inactivated
Please Note: An additional $10/bottle handling fee will be applied for 500 mL bottles.
Fetal Bovine Serum - Hybridoma Qualified, Heat Inactivated Summary
Key Benefits
• Supports clonal growth of hybridoma cells
• Screened for superior growth of hybridoma and myeloma cell lines
• Low endotoxin and low hemoglobin levels
• USDA-Approved FBS of Mexico/Central American origin
• Manufactured in our ISO 9001:2015 certified facility
• Formerly sold under the Atlanta Biologicals brand
Why Use Heat Inactivated Hybridoma Qualified Fetal Bovine Serum?
High quality Fetal Bovine Serum, also known as Fetal Calf Serum (FCS), is screened to identify lots that offer optimal performance with these cells. Hybridoma Qualified lots must show superior growth promotion, exceptional clonal growth at limiting dilution, and a lack of cytotoxicity during the growth studies. All lots of Hybridoma Qualified FBS are manufactured in our ISO 9001:2015 certified facility.
Heat Inactivation - Serum is inactivated by raising the temperature to 56 °C for 30 minutes under controlled conditions. The objective of heat inactivation is to destroy complement activity in the serum without affecting the growth-promoting characteristics of the product. Removal of complement activity from the serum is not required for most cell cultures, but may be necessary for cultures that are sensitive to the complement activity. Since heat inactivation of the serum may, to some extent, decrease the growth performance properties of the serum, this procedure should only be performed if actually required for optimal cell growth. Researchers should evaluate the applicability of heat inactivation in regards to their own application. If heat inactivation is required, the process should be carefully controlled to avoid increased formation of crystalline and flocculent precipitates, gelling of serum proteins and excessive loss of growth performance. Significant damage to serum can occur when it is subjected to higher than required temperatures or heated over extended lengths of time.
Protocol for Heat Inactivation of Serum Products.
Protocol for Fetal Bovine Serum Storage, Thawing, and Freezing.
Collection, Processing, and Testing of R&D Systems FBS
Collection and Processing - R&D Systems FBS is manufactured under a process that meets USDA requirements for animal products and ensures the product is traceable back to the date and location of collection. Our FBS manufacturing adheres to closed-system collection, processing in positive pressure, HEPA-filtered clean rooms, and triple 0.1 um filtration to remove contaminants. Strict control of the process from collection through processing enables us to provide a stable, consistent performing, and traceable supply of quality serum throughout global fluctuations in serum availability and regulatory requirements.
Virus Testing - Fetal Bovine Serum – Hybridoma Qualified undergoes testing for adventitious viruses, such as: Bovine Viral Diarrhea Virus (BVDV), Infectious Bovine Rhinotracheitis Virus (IBRV), Parainfluenza-3 Virus (PI-3V), Bluetongue Virus (BTV), Bovine Respiratory Syncytial Virus (BRSV), Bovine Parvovirus (BPV), Bovine Adenovirus, Type 3 (BAV-3), Bovine Adenovirus, Type 5 (BAV-5), Reo virus, and Rabies virus.
Biochemical Testing - R&D Systems FBS undergo biochemical profiling including total protein, albumin, globulin, IgG, total bilirubin, glucose, iron, cholesterol, triglycerides, sodium and potassium. View the product insert for a complete list of biochemical profiling components, which may vary between grades.
Chemical Analysis - Each lot of FBS is tested for osmolality, pH, hemoglobin, endotoxin (LAL method) and microbiological contamination (using methods recommended by the U.S. Pharmacopeia).
Specifications
Endotoxin - ≤ 50.0 EU/mL by Limulus Amebocyte Lysate (LAL) Assay
Hemoglobin - 25 mg/dL
Hybridoma Supports Hybridoma Cell Culture
Hormone Profile - Included
Limitations
For research use only. Not for diagnositic use.
Product Datasheets
FAQs
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What is the difference between Fetal Bovine Serum (FBS) and Fetal Calf Serum (FCS)?
There is no difference. These are different names for the same product. Serum derived from blood of bovine fetuses is referred to as Fetal Bovine Serum or Fetal Calf Serum.
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Should I use heat inactivated fetal bovine serum?
Heat inactivation, in general, decreases the growth promoting properties of the serum and causes increased formation of crystalline or flocculent precipitates (salts, proteins, lipids, fibrin, etc.) in the Fetal Bovine Serum (FBS) as well as in other serum products. In fact, for many cell culture applications, heat inactivation may be unnecessary. Warming the serum-containing medium to 37˚C prior to use, as is the practice in many cell culture laboratories, is often sufficient to inactivate heat-labile complement factors. Heat inactivated FBS is frequently used in immunological applications.
Since every cell type has different growth requirements, the choice of whether to heat inactivate your serum or not must be made by the researcher. If you have concerns about whether to use heat inactivated serum with your cell line or not, perform a side-by-side comparison of cell growth with heat inactivated versus non-heat inactivated serum.
For your convenience, R&D Systems offers all serum with or without heat-inactivation.
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