Fetal Bovine Serum - Hybridoma Qualified, Heat Inactivated

Fetal Bovine Serum - Hybridoma Qualified, Heat Inactivated Summary

Why Use Hybridoma Qualified Fetal Bovine Serum?

High quality Fetal Bovine Serum is screened to identify lots that offer optimal performance with these cells. Hybridoma Qualified lots must show superior growth promotion, exceptional clonal growth at limiting dilution, and a lack of cytotoxicity during the growth studies.

Why Heat Inactivate Serum?

The objective of heat inactivation is to destroy complement activity in the serum without affecting the growth-promoting characteristics of the product. Removal of complement activity from the serum is not required for most cell cultures, but may be necessary for cultures that are sensitive to the complement activity. Since heat inactivation of the serum may, to some extent, decrease the growth performance properties of the serum, this procedure should only be performed if actually required for optimal cell growth. Researchers should evaluate the applicability of heat inactivation in regards to their own application. If heat inactivation is required, the process should be carefully controlled to avoid increased formation of crystalline and flocculent precipitates, gelling of serum proteins and excessive loss of growth performance. Significant damage to serum can occur when it is subjected to higher than required temperatures or heated over extended lengths of time.

Specifications

Product Datasheets