Fetal Bovine Serum - Optima
Please Note: An additional $10/bottle handling fee will be applied for 500 mL bottles.
Fetal Bovine Serum - Optima Summary
• US-origin and USDA-Aphis certified
• Suitable for general cell culture applications
• High lot-to-lot consistency
• Excellent cell growth characteristics, low endotoxin, and low hemoglobin levels
• Manufactured in our ISO 9001:2015 certified facility
• Formerly sold under the Atlanta Biologicals brand
Why Use Fetal Bovine Serum - Optima?
Fetal Bovine Serum, also known as Fetal Calf Serum (FCS), is a critical supplement added to cell culture medium to maintain cell health and promote expansion. Different grades of FBS are available from R&D Systems that address the value and premium needs of research.
All lots of Fetal Bovine Serum – Optima are sterility, virus, and performance tested, and include a biochemical profile. Lots are manufactured from USA origin material following stringent manufacturing and quality control procedures. Optima FBS is USDA-APHIS certified and is manufactured in our ISO 9001:2015 certified facility.
Fetal Bovine Serum and other serum products should be stored and handled correctly to assure long-term stability and to preserve growth performance consistency throughout its shelf-life. In addition, heat inactivation of serum is frequently desired to inactivate complement within the serum. Below are protocol links for serum storage/handling and heat inactivation.
Protocol for Fetal Bovine Serum Storage, Thawing, and Freezing.
Protocol for Heat Inactivation of Serum Products.
Collection, Processing, and Testing of R&D Systems FBS
Collection and Processing - R&D Systems FBS is manufactured under a process that meets USDA requirements for animal products and ensures the product is traceable back to the date and location of collection. Our FBS manufacturing adheres to closed-system collection, processing in positive pressure, HEPA-filtered clean rooms, and triple 0.1 um filtration to remove contaminants. Strict control of the process from collection through processing enables us to provide a stable, consistent performing, and traceable supply of quality serum throughout global fluctuations in serum availability and regulatory requirements.
Virus Testing - Fetal Bovine Serum – Optima undergoes extended testing for adventitious viruses including: Bovine Viral Diarrhea Virus (BVDV), Infectious Bovine Rhinotracheitis Virus (IBRV), Parainfluenza-3 Virus (PI-3V), Bluetongue Virus (BTV), Bovine Respiratory Syncytial Virus (BRSV), Bovine Parvovirus (BPV), Bovine Adenovirus, Type 3 (BAV-3), Bovine Adenovirus, Type 5 (BAV-5), Reo virus, and Rabies virus.
Biochemical Testing - R&D Systems FBS undergo biochemical profiling including total protein, albumin, globulin, IgG, total bilirubin, glucose, iron, cholesterol, triglycerides, sodium and potassium. View the product insert for a complete list of biochemical profiling components, which may vary between grades.
Chemical Analysis - Each lot of FBS is tested for osmolality, pH, hemoglobin, endotoxin (LAL method) and microbiological contamination (using methods recommended by the U.S. Pharmacopeia).
Endotoxin - ≤ 20.0 EU/mL by Limulus Amebocyte Lysate (LAL) Assay
Hemoglobin - 25 mg/dL
For research use only. Not for diagnostic use.
Optima FBS has Consistent Lot-to-Lot Performance. Quality control data is displayed across multiple lots of Optima FBS. (A) Plating efficiency of cells cultured in media containing 5% or 10% Optima FBS, relative to control lots of FBS. (B) Optima FBS was tested for its ability to support hybridoma cloning as well as the healthy expansion of adherent and suspension cell culture lines. Lots of Optima FBS show consistent and high percentage cloning efficiency and cell viability.
Optima FBS Support Expansion and Health Across Cell Lines. Ba/F3 murine pro-B cell line, ATDC-5 murine teratocarcinoma cell line, and a TF-1 erythroleukemic cell line were evaluated for expansion and health in culture media supplemented with either Optima FBS or FBS from an alternate supplier (Suppler #1). (A) Two lots of Optima FBS showed consistent expansion rates for ATDC-5 cells. The expansion was similar to FBS from another supplier. (B) Ba/F3, ATDC-5, and TF-1 cell lines cultured in Optima FBS show consistent cell viability across multiple lots. The viability was comparable to an alternate supplier of FBS.
What is the difference between Fetal Bovine Serum (FBS) and Fetal Calf Serum (FCS)?
There is no difference. These are different names for the same product. Serum derived from blood of bovine fetuses is referred to as Fetal Bovine Serum or Fetal Calf Serum.
Should I use heat inactivated fetal bovine serum?
Heat inactivation, in general, decreases the growth promoting properties of the serum and causes increased formation of crystalline or flocculent precipitates (salts, proteins, lipids, fibrin, etc.) in the Fetal Bovine Serum (FBS) as well as in other serum products. In fact, for many cell culture applications, heat inactivation may be unnecessary. Warming the serum-containing medium to 37˚C prior to use, as is the practice in many cell culture laboratories, is often sufficient to inactivate heat-labile complement factors. Heat inactivated FBS is frequently used in immunological applications.
Since every cell type has different growth requirements, the choice of whether to heat inactivate your serum or not must be made by the researcher. If you have concerns about whether to use heat inactivated serum with your cell line or not, perform a side-by-side comparison of cell growth with heat inactivated versus non-heat inactivated serum.
For your convenience, R&D Systems offers all serum with or without heat-inactivation.
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