Fetal Bovine Serum - TET Tested

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Catalog # Availability Size / Price Qty
S10310
S10350
S10395
Fetal Bovine Serum - TET Tested
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Fetal Bovine Serum - TET Tested Summary

TET Tested Fetal Bovine Serum is specifically designed for use in tetracycline/tetracycline derivatives-regulated gene expression systems in cultured cells.

Key Benefits

• FBS with undetectable levels of tetracycline or tetracycline derivatives
• Specifically formulated for cell lines using TET-driven gene expression
• Excellent cell growth characteristics, low endotoxin, and low hemoglobin levels
• USDA-Approved FBS of Mexico/Central American origin
• Manufactured in our ISO 9001:2015 certified facility
• Formerly sold under the Atlanta Biologicals brand

Why Use TET Tested Fetal Bovine Serum?

Fetal Bovine Serum, also known as Fetal Calf Serum (FCS), is a critical supplement added to cell culture medium to maintain cell health and promote expansion. Different grades of FBS are available from R&D Systems that address the value and premium needs of research.

TET Tested Fetal Bovine Serum is specifically designed for use in tetracycline / tetracycline derivatives-regulated gene expression systems in cultured cells. The tetracycline and tetracycline derivative contents of high quality FBS is measured, and only lots with undetectable levels are selected as TET Tested Fetal Bovine Serum. All lots of TET Tested FBS are manufactured in our ISO 9001:2015 certified facility.

Fetal Bovine Serum and other serum products should be stored and handled correctly to assure long-term stability and to preserve growth performance consistency throughout its shelf-life. In addition, heat inactivation of serum is frequently desired to inactivate complement within the serum. Below are protocol links for serum storage/handling and heat inactivation.

Protocol for Fetal Bovine Serum Storage, Thawing, and Freezing.
Protocol for Heat Inactivation of Serum Products.

Collection, Processing, and Testing of R&D Systems FBS

Collection and Processing - R&D Systems FBS is manufactured under a process that meets USDA requirements for animal products and ensures the product is traceable back to the date and location of collection. Our FBS manufacturing adheres to closed-system collection, processing in positive pressure, HEPA-filtered clean rooms, and triple 0.1 um filtration to remove contaminants. Strict control of the process from collection through processing enables us to provide a stable, consistent performing, and traceable supply of quality serum throughout global fluctuations in serum availability and regulatory requirements.

Virus Testing - Fetal Bovine Serum – TET Tested undergoes testing for adventitious viruses, such as: Bovine Viral Diarrhea Virus (BVDV), Infectious Bovine Rhinotracheitis Virus (IBRV), Parainfluenza-3 Virus (PI-3V), Bluetongue Virus (BTV), Bovine Respiratory Syncytial Virus (BRSV), Bovine Parvovirus (BPV), Bovine Adenovirus, Type 3 (BAV-3), Bovine Adenovirus, Type 5 (BAV-5), Reo virus, and Rabies virus.

Biochemical Testing - R&D Systems FBS undergo biochemical profiling including total protein, albumin, globulin, IgG, total bilirubin, glucose, iron, cholesterol, triglycerides, sodium and potassium. View the product insert for a complete list of biochemical profiling components, which may vary between grades.

Chemical Analysis - Each lot of FBS is tested for osmolality, pH, hemoglobin, endotoxin (LAL method) and microbiological contamination (using methods recommended by the U.S. Pharmacopeia).

Specifications

Osmolality
280 -335 mOsm
Purification
Triple 0.1 μm filtered
Sterility Testing
Pass
Testing Cell Culture
Pass
Testing Additional
pH - 6.8-7.8

Endotoxin - ≤ 50.0 EU/mL by Limulus Amebocyte Lysate (LAL) Assay

Hemoglobin - 25 mg/dL
TET Testing - Tetracycline, Chlorotetracycline, Oxytetracycline, and Doxycycline
Shipping Conditions
On Dry Ice
Storage
-5 to -20 °C
Applications
Cell Culture
Species
Bovine

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Data Example

Fetal Bovine Serum - TET Tested

Reagents Provided

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