FGF acidic/FGF1 Antibody (3X3E5)

Novus Biologicals | Catalog # NBP3-16609

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3X3E5 expressed in HEK293
View all FGF acidic/FGF1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 56-155 of human FGF acidic/FGF1 (P05230). HIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FGF acidic/FGF1 Antibody (3X3E5)

Western Blot: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609]

Western Blot: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609]

Western Blot: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609] - Western blot analysis of extracts of various cell lines, using FGF acidic/FGF1 Rabbit mAb (NBP3-16609) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
FGF acidic/FGF1 Antibody (3X3E5)

Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609] -

Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609] - Confocal imaging of NIH/3T3 cells using FGF acidic/FGF1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
FGF acidic/FGF1 Antibody (3X3E5)

Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609] -

Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609] - Confocal imaging of U-2 OS cells using FGF acidic/FGF1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for FGF acidic/FGF1 Antibody (3X3E5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FGF acidic/FGF1

FGF acidic/FGF1, also known as ECGF and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF1 is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF1 with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF1 can translocate to the cytosol and to the nucleus. Intracellular FGF1 functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.

Long Name

Fibroblast Growth Factor acidic

Alternate Names

alpha-ECGF, beta-ECGF, FGF acidic, FGF-1, HBGF-1

Gene Symbol

FGF1

Additional FGF acidic/FGF1 Products

Product Documents for FGF acidic/FGF1 Antibody (3X3E5)

Certificate of Analysis

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Product Specific Notices for FGF acidic/FGF1 Antibody (3X3E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for FGF acidic/FGF1 Antibody (3X3E5)

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  • Q: What is the heparin binding activity of FGF and VEGF?

    A: These proteins are not assayed for their ability to bind Heparin. More information about the FGF family of growth factors is available in this review article: Basilico, C. (1992) Adv. Can. Res. 59:115.

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