FGF acidic/FGF1, also known as ECGF and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF1 is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF1 with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF1 can translocate to the cytosol and to the nucleus. Intracellular FGF1 functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.
FGF acidic/FGF1 Antibody (V8P1G5*F2) - BSA Free
Novus Biologicals | Catalog # NBP2-50406
![Immunohistochemistry: FGF acidic/FGF1 Antibody (V8P1G5*F2) - BSA Free [NBP2-50406]](https://resources.rndsystems.com/images/products/FGF-acidic-FGF1-Antibody-V8P1G5F2-Immunohistochemistry-NBP2-50406-img0001.jpg "Immunohistochemistry: FGF acidic/FGF1 Antibody (V8P1G5*F2) - BSA Free [NBP2-50406]")
Key Product Details
Species Reactivity
Zebrafish
Applications
Immunohistochemistry, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # V8P1G5*F2
Format
BSA Free
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Product Specifications
Immunogen
Ovalbumin-conjugated synthetic peptide LPRQVEQEED (C-terminal sequence)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The clone number is listed as V8P1G5*F2 in the scientific literature.
Scientific Data Images for FGF acidic/FGF1 Antibody (V8P1G5*F2) - BSA Free
Immunohistochemistry: FGF acidic/FGF1 Antibody (V8P1G5*F2) - BSA Free [NBP2-50406]
Immunohistochemistry: FGF acidic/FGF1 Antibody (V8P1G5F2) [NBP2-50406] - Whole-mount zebrafish embryoApplications for FGF acidic/FGF1 Antibody (V8P1G5*F2) - BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunohistochemistry
1:10 - 1:500
Application Notes
Positive control(s): ELISA - peptide immunogen; IHC - zebrafish embryo.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FGF acidic/FGF1
Long Name
Fibroblast Growth Factor acidic
Alternate Names
alpha-ECGF, beta-ECGF, FGF acidic, FGF-1, HBGF-1
Gene Symbol
FGF1
Additional FGF acidic/FGF1 Products
Product Documents for FGF acidic/FGF1 Antibody (V8P1G5*F2) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FGF acidic/FGF1 Antibody (V8P1G5*F2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for FGF acidic/FGF1 Antibody (V8P1G5*F2) - BSA Free
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Q: What is the heparin binding activity of FGF and VEGF?
A: These proteins are not assayed for their ability to bind Heparin. More information about the FGF family of growth factors is available in this review article: Basilico, C. (1992) Adv. Can. Res. 59:115.
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