Fibrinogen Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-50087

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Immunohistochemistry, Western Blot, Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Fibrinogen Primary Antibodies »

Product Specifications

Immunogen

Fibrinogen [Human Plasma] (Uniprot: P02671)

Reactivity Notes

Cross reactivity against Fibrinogen from other sources is unknown

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Fibrinogen [Human Plasma]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Fibrinogen Antibody - BSA Free

Western Blot: Fibrinogen Antibody [NBP2-50087]

Western Blot: Fibrinogen Antibody [NBP2-50087]

Western Blot: Fibrinogen Antibody [NBP2-50087] - Lane 1: Fibrinogen under reducing conditions. Lane 2: none. Load: 1 ug per lane. Primary antibody: Fibrinogen antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: TBS/blocking buffer @ 1 hr RT.
Immunohistochemistry-Paraffin: Fibrinogen Antibody [NBP2-50087]

Immunohistochemistry-Paraffin: Fibrinogen Antibody [NBP2-50087]

Immunohistochemistry-Paraffin: Fibrinogen Antibody [NBP2-50087] - Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Fibrinogen antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibrinogen is localized in plasma. Staining: Fibrinogen as precipitated red signal with hematoxylin purple nuclear counterstain.
Fibrinogen Antibody

Fibrinogen Antibody

Immunohistochemistry of Goat Anti-Fibrinogen antibody. Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Fibrinogen antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibrinogen is localized in plasma. Staining: Fibrinogen as precipitated red signal with hematoxylin purple nuclear counterstain.
Fibrinogen Antibody

Fibrinogen Antibody

Western Blot of Fibrinogen Antibody. Lane 1: Molecular Weight Ladder. Lane 2: Fibrinogen 0.05ug. Primary Antibody: Anti-Fibrinogen 1:4000 overnight at 2-8C. Secondary Antibody: Donkey Anti-Goat IgG HRP at 1:40,000 for 30min at RT. Block: BlockOut Buffer

Applications for Fibrinogen Antibody - BSA Free

Application
Recommended Usage

ELISA

1:20000-1:100000

Immunohistochemistry

1:500

Immunohistochemistry-Paraffin

1:500

Western Blot

1:2000-1:10000
Application Notes
This product has been tested in western blot and immunohistochemistry. This antibody is suitable when assayed against 1.0 ug of Fibrinogen [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fibrinogen

Alternate Names

Factor I, HEL-S-78p

Gene Symbol

FGA

UniProt

P02671

Additional Fibrinogen Products

Product Documents for Fibrinogen Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Fibrinogen Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fibrinogen Antibody - BSA Free

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Protocols

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