Fibrinogen Antibody
Novus Biologicals | Catalog # NBP2-80414
![Western Blot: Fibrinogen Antibody [NBP2-80414]](https://beta-resources.rndsystems.com/images/products/Fibrinogen-Antibody-Western-Blot-NBP2-80414-img0004.jpg "Western Blot: Fibrinogen Antibody [NBP2-80414]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Native protein. (SwissProt: P02671; SwissProt: P0267 Human 5; SwissProt: P02679 Human)
Localization
Secreted.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Fibrinogen Antibody (NBP2-80414) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-Fibrinogen Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Fibrinogen Antibody
Western Blot: Fibrinogen Antibody [NBP2-80414]
Western Blot: Fibrinogen Antibody [NBP2-80414] - Analysis of Fibrinogen on mouse bone marrow and human kidney tissue lysates using anti-Fibrinogen antibody at 1/500 dilution.![Immunocytochemistry/ Immunofluorescence: Fibrinogen Antibody [NBP2-80414]](https://beta-resources.rndsystems.com/images/products/Fibrinogen-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-80414-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Fibrinogen Antibody [NBP2-80414]")
Immunocytochemistry/ Immunofluorescence: Fibrinogen Antibody [NBP2-80414]
Immunocytochemistry/Immunofluorescence: Fibrinogen Antibody [NBP2-80414] - Staining Fibrinogen in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.![Flow Cytometry: Fibrinogen Antibody [NBP2-80414]](https://beta-resources.rndsystems.com/images/products/Fibrinogen-Antibody-Flow-Cytometry-NBP2-80414-img0001.jpg "Flow Cytometry: Fibrinogen Antibody [NBP2-80414]")
Flow Cytometry: Fibrinogen Antibody [NBP2-80414]
Flow Cytometry: Fibrinogen Antibody [NBP2-80414] - Analysis of NIH-3T3 cells with Fibrinogen antibody at 1/100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.![Immunocytochemistry/ Immunofluorescence: Fibrinogen Antibody [NBP2-80414]](https://beta-resources.rndsystems.com/images/products/Fibrinogen-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-80414-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Fibrinogen Antibody [NBP2-80414]")
Immunocytochemistry/ Immunofluorescence: Fibrinogen Antibody [NBP2-80414]
Immunocytochemistry/Immunofluorescence: Fibrinogen Antibody [NBP2-80414] - Staining Fibrinogen in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.Applications for Fibrinogen Antibody
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Western Blot
1:500
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.2% BSA, 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fibrinogen
Alternate Names
Factor I
Gene Symbol
FGA
Additional Fibrinogen Products
Product Documents for Fibrinogen Antibody
Product Specific Notices for Fibrinogen Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Fibrinogen Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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