Fibrinogen gamma chain Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32436

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Fibrinogen gamma chain Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fibrinogen gamma chain. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (84%)

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fibrinogen gamma chain Antibody - BSA Free

Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436]

Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436]

Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436] - Mouse plasma and rat plasma (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Fibrinogen gamma chain Antibody [NBP1-32436]

Immunocytochemistry/ Immunofluorescence: Fibrinogen gamma chain Antibody [NBP1-32436]

Immunocytochemistry/Immunofluorescence: Fibrinogen gamma chain Antibody [NBP1-32436] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fibrinogen gamma protein stained by Fibrinogen gamma antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Fibrinogen gamma chain Antibody [NBP1-32436]

Immunohistochemistry-Paraffin: Fibrinogen gamma chain Antibody [NBP1-32436]

Immunohistochemistry-Paraffin: Fibrinogen gamma chain Antibody [NBP1-32436] - Breast cancer. Fibrinogen gamma antibody dilution: 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436]

Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436]

Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted at 1:500.
Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436]

Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436]

Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436] - Human plasma and HepG2 conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunoprecipitation: Fibrinogen gamma chain Antibody [NBP1-32436]

Immunoprecipitation: Fibrinogen gamma chain Antibody [NBP1-32436]

Immunoprecipitation: Fibrinogen gamma chain Antibody [NBP1-32436] - Fibrinogen gamma protein from HepG2 whole cell extracts using 5 ug of Fibrinogen gamma antibody. Western blot analysis was performed using Fibrinogen gamma antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Fibrinogen gamma chain Antibody

Immunocytochemistry/ Immunofluorescence: Fibrinogen gamma chain Antibody [NBP1-32436] -

Immunocytochemistry/ Immunofluorescence: Fibrinogen gamma chain Antibody [NBP1-32436] - Fibrinogen gamma chain antibody detects Fibrinogen gamma chain protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fibrinogen gamma chain stained by Fibrinogen gamma chain antibody (NBP1-32436) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Scale bar= 10um.
Fibrinogen gamma chain Antibody - BSA Free

Western Blot: Fibrinogen gamma chain Antibody [NBP1-32436] -

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody (NBP1-32436) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Fibrinogen gamma chain Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Fibrinogen gamma chain

The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms.

Alternate Names

fibrinogen gamma chain, fibrinogen, gamma polypeptide

Gene Symbol

FGG

Additional Fibrinogen gamma chain Products

Product Documents for Fibrinogen gamma chain Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Fibrinogen gamma chain Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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