Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # ER-TR7

Format

BSA Free
View all Fibroblast Primary Antibodies »

Product Specifications

Immunogen

Isolated C3H thymic stromal cells

Marker

Fibroblast Marker

Specificity

NB100-64932 recognizes ER-TR7, an antigen that is located in the cytoplasm of reticular fibroblasts and is a component of the extracellular matrix of lymphoid and non-lymphoid organs. The antigen recognized by clone ER-TR7 has not been identified but studies suggest that it is likely to be distinct from laminin, fibronectin, collagen types I-IV, heparin sulphate proteoglycan, entactin and nidogen. Clone ER-TR7 has been used to stain the microanatomy of various organs and also stains subendothelial deposits in atherosclerotic plaques.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Fibroblast Antibody (ER-TR7) - BSA Free

Immunohistochemistry: Fibroblast Antibody (ER-TR7) - BSA Free [NB100-64932]

Immunohistochemistry: Fibroblast Antibody (ER-TR7) - BSA Free [NB100-64932]

Fibroblast-Antibody-ER-TR7-Immunohistochemistry-NB100-64932-img0002.jpg
Immunohistochemistry-Frozen: Fibroblast Antibody (ER-TR7) - BSA Free [NB100-64932]

Immunohistochemistry-Frozen: Fibroblast Antibody (ER-TR7) - BSA Free [NB100-64932]

Immunohistochemistry-Frozen: Fibroblast Antibody (ER-TR7) [NB100-64932] - Fibroblast Antibody (ER-TR7) IHC on Mouse lymph node, frozen section, 20x. Primary antibody diluted 1:500. This image was submitted via customer Review.

Applications for Fibroblast Antibody (ER-TR7) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:300-1:500. Use reported in scientific literature (PMID 22042977)

Immunohistochemistry-Frozen

1:500

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 35794106)
Application Notes
Membrane permeabilisation is required for Flow Cytometry.

Reviewed Applications

Read 1 review rated 4 using NB100-64932 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibroblast

Connective tissue consists of a ground of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts. This monoclonal antibody [ER-TR7] detects an antigen present in, and produced by, reticular fibroblasts. The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The antibody also stains subendothelial deposits in the plaque area of atherosclerotic plaques.

Alternate Names

reticular fibroblast

Additional Fibroblast Products

Product Documents for Fibroblast Antibody (ER-TR7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Fibroblast Antibody (ER-TR7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fibroblast Antibody (ER-TR7) - BSA Free

Customer Reviews for Fibroblast Antibody (ER-TR7) - BSA Free (1)

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Customer Images

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  • Fibroblast Antibody (ER-TR7)
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: PFA Fixed Frosen Section Mouse Lymphnode
    Species: Mouse
    Verified Customer | Posted 06/29/2017
    Fibroblast Antibody (ER-TR7) IHC on Mouse lymph node, frozen section, 20x
    WD [1:500]; secondary ab RTU Polymer-HRP
    Fibroblast Antibody (ER-TR7) - BSA Free NB100-64932

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Protocols

View specific protocols for Fibroblast Antibody (ER-TR7) - BSA Free (NB100-64932):


Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.


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FAQs for Fibroblast Antibody (ER-TR7) - BSA Free

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  • Q: I am currently in search of an antibody for staining of human dermal fibroblasts. Can you recommend me an antibody for this? Is there an antibody that specifically stains only fibroblasts and not mesenchymal stem cells?

    A:

    We sell a number of antibodies which are suitable for staining fibroblasts. You can see these at the following links: NB100-64932, NB110-15846 and NB100-1845. NB100-64932 recognizes ER-TR7, an antigen that is located in the cytoplasm of reticular fibroblasts and is a component of the extracellular matrix of lymphoid and non-lymphoid organs. The antigen recognized by clone ER-TR7 has not been identified but studies suggest that it is likely to be distinct from laminin, fibronectin, collagen types I-IV, heparin sulphate proteoglycan, entactin and nidogen. NB110-15846 is specific to beta subunit of prolyl 4-hydroxylase. It reacts with fibroblasts and myoepithelial cells, and does not stain lymphocytes, monocytes, dendritic cells or granulocytes. NB100-1845 recognizes an epitope on primary cultured human fibroblasts, fibroblast cell lines, tissue macrophages and 95% of peripheral blood monocytes. It does not react with vascular smooth muscle cells. Unfortunately, we do not have any data regarding the staining of mesenchymal stem cells with any of these antibodies in our QC lab.

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