Fibroblasts Antibody (TE-7)

Novus Biologicals | Catalog # NBP2-50082

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TE-7
View all Fibroblasts Primary Antibodies »

Product Specifications

Immunogen

Whole human thymic stoma cells (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168).

Specificity

The antibody reacts with fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen defined by antibody TE-7 appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue, e.g. fibrosarcomas (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168). The antibody is useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Fibroblasts Antibody (TE-7)

Immunocytochemistry/ Immunofluorescence: Fibroblasts Antibody (TE-7) [NBP2-50082]

Immunocytochemistry/ Immunofluorescence: Fibroblasts Antibody (TE-7) [NBP2-50082]

Immunocytochemistry/Immunofluorescence: Fibroblasts Antibody (TE-7) [NBP2-50082] - MRC-5 human embryonic lung fibroblast cell line, fixation with PFA 4%, blocking with PBS 1X + 1% BSA + 0.1% Tween, primary antibody used at 1:50 in blocking buffer, O/N. Secondary antibody: goat anti mouse Alexa Fluor 594 for 1 hour at RT. Signal was detected by fluorescence microscopy at 40X. This image was submitted via customer review.
Immunohistochemistry-Paraffin: Fibroblasts Antibody (TE-7) [NBP2-50082]

Immunohistochemistry-Paraffin: Fibroblasts Antibody (TE-7) [NBP2-50082]

Immunohistochemistry-Paraffin: Fibroblasts Antibody (TE-7) [NBP2-50082] - Paraffin-embedded gastrocnemius section from a patient with myopathic condition was performed overnight at 4 degrees C using 10 ug/mL.

Applications for Fibroblasts Antibody (TE-7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Reviewed Applications

Read 1 review rated 5 using NBP2-50082 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.07% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibroblasts

Alternate Names

fibro-blasts

Additional Fibroblasts Products

Product Documents for Fibroblasts Antibody (TE-7)

Certificate of Analysis

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Product Specific Notices for Fibroblasts Antibody (TE-7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fibroblasts Antibody (TE-7)

Customer Reviews for Fibroblasts Antibody (TE-7) (1)

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Customer Images

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  • Fibroblasts Antibody (TE-7)
    Name: Maxime Bourrelle-Langlois
    Application: Immunocytochemistry
    Sample Tested: MRC-5 human embryonic lung fibroblast cell line
    Species: Human
    Verified Customer | Posted 03/22/2018
    MRC-5 human embryonic lung fibroblast cells
    MRC-5 human embryonic lung fibroblast cell line, fixation with PFA 4%, blocking with PBS 1X + BSA 1% + 0.1% Tween, primary antibody used at 1:50 in blocking buffer, O/N
    Fibroblasts Antibody (TE-7) NBP2-50082

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FAQs

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