Fibronectin Antibody (CL3730) - BSA Free

Novus Biologicals | Catalog # NBP2-61633

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3730

Format

BSA Free
View all Fibronectin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P02751, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Fibronectin Antibody (CL3730) - BSA Free

Western Blot: Fibronectin Antibody (CL3730) [NBP2-61633]

Western Blot: Fibronectin Antibody (CL3730) [NBP2-61633]

Western Blot: Fibronectin Antibody (CL3730) [NBP2-61633] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line U-87 MG
Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human pancreas shows absence of immunoreactivity in glandular cells (negative control).
Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human endometrium shows strong immunoreactivity in endometrial stroma and basement membrane of glandular cells.
Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human placenta shows strong immunoreactivity in chorionic villi.
Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human testis shows immunoreactivity in the connective tissue surrounding seminiferous tubules.
Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human prostate shows moderate positivity in smooth muscle.
Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human small intestine shows strong immunoreactivity in the connective tissue.
Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human breast cancer shows strong immunoreactivity in tumor stroma.
Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human liver cancer shows strong positivity in tumor.

Applications for Fibronectin Antibody (CL3730) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibronectin

Fibronectin (also known as FN1, or Fibronectin 1) is an extracellular matrix glycoprotein that binds to integrins and has a large role in cell adhesion, migration, differentiation, and growth. It plays a key role in wound healing by helping form blood clots and participates in embryonic development by guiding cell attachment and migration. Fibronectin consists of two monomers linked by disulfide bonds and binds extracellular components such as collagen fibrin and syndecans. Fibronectin has been implicated in carcinoma and tumor development while also limiting tumor cells response to treatment. Novus offers Fibronectin antibodies, ELISA Kits, lysates, and proteins

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Gene Symbol

FN1

Additional Fibronectin Products

Product Documents for Fibronectin Antibody (CL3730) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Fibronectin Antibody (CL3730) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Fibronectin Antibody (CL3730) - BSA Free

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  • Q: What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?

    A: Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application. 

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