Fibronectin Antibody

Novus Biologicals | Catalog # NBP1-31369

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Fibronectin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Fibronectin. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken 88%.

Localization

Secreted, extracellular space, extracellular matrix

Marker

Mesenchymal Cells Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

263 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Fibronectin Antibody (NBP1-31369) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Fibronectin Antibody

Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody [NBP1-31369]

Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody [NBP1-31369]

Immunocytochemistry/Immunofluorescence: Fibronectin/Anastellin Antibody [NBP1-31369] - Analysis of HeLa, using NBP1-31369 at 1:200 dilution.
Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP1-31369]

Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP1-31369]

Immunohistochemistry-Paraffin: Fibronectin/Anastellin Antibody [NBP1-31369] - Paraffin-embedded hepatoma. Fibronectin antibody [N1N2], N-term dilution: 1:500.
Western Blot: Fibronectin Antibody [NBP1-31369]

Western Blot: Fibronectin Antibody [NBP1-31369]

Western Blot: Fibronectin/Anastellin Antibody [NBP1-31369] - Mouse tissue extracts (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] diluted at 1:1000.
Immunoprecipitation: Fibronectin Antibody [NBP1-31369]

Immunoprecipitation: Fibronectin Antibody [NBP1-31369]

Immunoprecipitation: Fibronectin/Anastellin Antibody [NBP1-31369] - N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of Fibronectin by 3 ug of Fibronectin antibody [N1N2].
ELISA: Fibronectin Antibody [NBP1-31369]

ELISA: Fibronectin Antibody [NBP1-31369]

ELISA: Fibronectin Antibody [NBP1-31369] - Indirect ELISA analysis was performed by coating plate with 100 uL of recombinant Fibronectin protein at concentration of 10 ug/mL. The coated protein is detected with Fibronectin antibody [N1N2], N-term at rangeing from 0.5 to 140 ng/mL.
ELISA: Fibronectin Antibody [NBP1-31369]

ELISA: Fibronectin Antibody [NBP1-31369]

ELISA: Fibronectin Antibody [NBP1-31369] - Competitive ELISA detection of human fibronectin in plasma. Recombinant or purified native Fibronectin protein was coated to an ELISA plate at concentration of 10 ug/ml. Human plasma was applied as the competitors and was serial diluted at 1, 20, 200, 2000, 20000, 200000X dilutions. The primary antibody concentration was diluted to 14 ng/mL. Rabbit IgG antibody (HRP) was diluted at 1:2,000 and used to detect the primary antibody
Fibronectin Antibody

Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP1-31369] -

Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP1-31369] - Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
Fibronectin stained by Fibronectin antibody [N1N2], N-term (NBP1-31369) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Fibronectin Antibody

Western Blot: Fibronectin Antibody [NBP1-31369] -

Western Blot: Fibronectin Antibody [NBP1-31369] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (NBP1-31369) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Fibronectin Antibody

Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP1-31369] -

Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP1-31369] - Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Fibronectin stained by Fibronectin antibody [N1N2], N-term (NBP1-31369) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Fibronectin Antibody

Western Blot: Fibronectin Antibody [NBP1-31369] -

Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (NBP1-31369) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Fibronectin Antibody

Western Blot: Fibronectin Antibody [NBP1-31369] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (NBP1-31369) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Fibronectin Antibody

Application
Recommended Usage

ELISA

1:1000-1:10000

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Fibronectin

Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities.

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Gene Symbol

FN1

Additional Fibronectin Products

Product Documents for Fibronectin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Fibronectin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Fibronectin Antibody

Showing  1 - 2 of 2 FAQs Showing All

    • Q: For NBP1-31369 the Fibronectin antibody, do I need to add any other buffer solution before aliquotting it?
      A: For the Fibronectin antibody (NBP1-31369) you do not need to add anything else to the buffer to store it in your aliquots. It already has glycerol, which will keep it stable at lower temperatures and the main thing is to avoid repeated freeze thaw cycles that might cause your antibody to lose binding affinity.

    • Q: What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?
      A: Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application. 
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